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蒙古口蘑多糖提取工艺的研究 被引量:6

Optimization of Polysaccharide Extraction from Tricholoma Mongolicum Imai.
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摘要 以蒙古口蘑(Tricholoma mongolicumImai.)野生子实体和人工液体培养的菌丝体为研究对象,采用常规热水浸提法,对影响多糖提取得率的因素以正交试验的方法进行优化。结果表明,蒙古口蘑子实体多糖提取的最佳工艺为:提取时间4 h,提取4次,提取温度70℃,醇析浓度80%,料液比1∶10,经此工艺子实体的多糖得率为5.78%;菌丝体多糖提取最优工艺为:提取时间3 h,提取3次,提取温度90℃,醇析浓度80%,料液比1∶10,经此工艺菌丝体的多糖得率为5.93%。 Orthogonal experiments were applied to optimize the polysaecharide extraction from fruiting bodies and mycelium of Tricholoma mongolicum Imai. respectively. The optimal extraction parameters for fruiting bodies were predicted as followed: extraction temperature was 70℃, the ratio of raw material and water was 1:10, extraction time was 4 hours, extraction was done for 4 times and by 80% (V/V) of ethanol for polysaccharide precipitation. With this technics, the yield of polysaccharides was 5.78%. The optimum extraction conditions for mycelium were extracted at temperature of 90℃, with ratio between raw material and water as 1:10. The extract would last for 3 hours, and be made for 3 times and by 80% ethanol for polysaccharide precipitation. With this technics, the yield of polysaccharides was 5.93%.
出处 《中国农业科技导报》 CAS CSCD 2009年第4期129-132,共4页 Journal of Agricultural Science and Technology
基金 内蒙古农业大学博士基金项目(k13625)资助
关键词 蒙古口蘑 多糖 提取工艺 Tricholoma mongolicum Imai. polysaccharide extraction technics
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