摘要
根据GenBank已发表的传染性支气管炎病毒（IBV）全基因组序列设计引物，对793／B型IBV分离毒株TA03M基因进行克隆与序列分析。结果表明，793／B型IBV的M基因由669bp组成，与GenBank已发表的15株IBV的M基因相比较，793／B型IBV的M基因在第4-15位发生了3-12个核苷酸的缺失，对应1-4个氨基酸的缺失，共有30多处点突变。与其他各毒株M基因的核苷酸同源性为84.2％-93.6％，氨基酸同源性为82.1％-96.0％；进化分析显示TA03株与H52和IBN毒株之间的亲缘关系较近。该研究为进一步探讨793／B型IBVM基因（蛋白）在遗传变异和免疫等方面的作用奠定了理论基础。
According to the genomic sequences of IBV published in GenBank, a pair of primers was designed for amplifying the M gene of IBV of 793/B serotype by RT-PCR. The result of sequence analysis showed that the whole M gene of isolated IBV consisted of 669 nucleotides. Compared with 15 IBV strains which were puhlished in Gen- Bank,3-12 nucleotides were absent in 4-15 position （1-4 corresponding aminoacids absence） and about 30 point mutations were found in the M gene. The homology of nucleotide sequences was 84.20/00-93.6% ,and the homology of deduced aminoacids was 82.1%-96.0%. Evolution analysis indicated that the TA03 strain was closely related to H52 and IBN strains. The results provided a theoretical foundation for further research on the function of M gene （protein） in the genetic variation and immunization.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第11期1377-1381,共5页
Chinese Journal of Veterinary Science
基金
山东省科技攻关项目(2007GG30009001)
