摘要
目的建立一种快速的多重聚合酶链反应(PCR)方法检测鲍氏不动杆菌D类碳青酶烯酶基因。方法根据blaOXA-51、blaOXA-23的保守区设计引物,建立检测D类碳青酶烯酶blaOXA-51、blaOXA-23基因的多重PCR方法。结果30株鲍氏不动杆菌中有24株同时扩出blaOXA-51和blaOXA-23,另外6株只扩出blaOXA-51条带。结论实验所建立的多重PCR能快速检测鲍氏不动杆菌D类碳青酶烯酶基因。
OBJECTIVE To establish a rapid method of detecting class D carbapenemase gene in Acinetobacter baumannii by multiplex PCR. METHODS Design primers according to the conservative region of blaOXA-51 and blaOXA-23 , the class D carbapenemase gene were detected by Multiplex PCR. RESULTS 24 of 30 A. baumannii isolates produced two bands of blaOXA-51 and blaOXA-23 ; other 6 isolates showed a band of blaOXA-23. CONCLUSIONS The multiplex PCR could detect rapidly the class D carbapenemase gene.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第21期2824-2825,共2页
Chinese Journal of Nosocomiology
