摘要
目的探讨齐墩果酸对顺铂耐药胃癌SGC-7901细胞增殖的影响及其作用机制。方法体外诱导建立人胃癌顺铂耐药细胞株SGC-7901/CDDP,MTT比色法检测SGC-7901/CDDP细胞活力;适时荧光定量PCR分析凋亡相关基因Bcl-2及BaxmRNA的表达。结果MTT实验证明齐墩果酸对SGC-7901/CDDP细胞的生长有抑制作用,100μmol.L-1齐墩果酸作用72h对SGC-7901/CDDP细胞的抑制率达62%,并表现为时间和剂量依赖性;适时荧光定量PCR实验证明,SGC-7901/CDDP细胞经齐墩果酸处理后,促凋亡基因Bax表达上调,抗凋亡基因Bcl-2表达下调。结论齐墩果酸在体外能够抑制SGC-7901/CDDP细胞增殖,并使促凋亡基因Bax表达升高,抗凋亡基因Bcl-2表达降低,上调Bax和下调Bcl-2mRNA的表达可能是其作用机制之一。
Aim To investigate the effects of oleanolic acid on proliferation of SGC-7901/CDDP cells in vitro and its potential mechanisms. Methods Model of SGC-7901 cells resistant to cisplatin (SGC-7901/CD- DP) was established in vitro. MTT assay was used to e- valuate the viability of SGC-7901/CDDP cells in vitro. Real-time PCR was used to analyse the expression of Bel-2 and Bax gene. Results MTT assay showed that oleanolie acid dramatically inhibited the proliferation of SGC-7901/CDDP cells in vitro, more than 62% SGC- 7901/CDDP cells were inhibited when the cells were treated with 100 μmol · L^-1 oleanolic acid for 72 h; Real-time PCR showed that Bax (pro-apoptotic gene) was upregulated and Bcl-2 (anti-apoptotic)was downregulated after being treated with oleanolic acid. Conclusions These results suggest that oleanolic acid inhibits proliferation of SGC-7901/CDDP cells in vitro, and its potential mechanisms may be through the upregulation of Bax and the downregulation of Bcl-2.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第10期1334-1337,共4页
Chinese Pharmacological Bulletin
基金
黑龙江省教育厅资助项目(No11531439)