期刊文献+

齐墩果酸对顺铂耐药胃癌SGC-7901细胞增殖的影响及其机制研究 被引量:25

Effects of oleanolic acid on proliferation of SGC-7901/CDDP in vitro and its potential mechanisms
下载PDF
导出
摘要 目的探讨齐墩果酸对顺铂耐药胃癌SGC-7901细胞增殖的影响及其作用机制。方法体外诱导建立人胃癌顺铂耐药细胞株SGC-7901/CDDP,MTT比色法检测SGC-7901/CDDP细胞活力;适时荧光定量PCR分析凋亡相关基因Bcl-2及BaxmRNA的表达。结果MTT实验证明齐墩果酸对SGC-7901/CDDP细胞的生长有抑制作用,100μmol.L-1齐墩果酸作用72h对SGC-7901/CDDP细胞的抑制率达62%,并表现为时间和剂量依赖性;适时荧光定量PCR实验证明,SGC-7901/CDDP细胞经齐墩果酸处理后,促凋亡基因Bax表达上调,抗凋亡基因Bcl-2表达下调。结论齐墩果酸在体外能够抑制SGC-7901/CDDP细胞增殖,并使促凋亡基因Bax表达升高,抗凋亡基因Bcl-2表达降低,上调Bax和下调Bcl-2mRNA的表达可能是其作用机制之一。 Aim To investigate the effects of oleanolic acid on proliferation of SGC-7901/CDDP cells in vitro and its potential mechanisms. Methods Model of SGC-7901 cells resistant to cisplatin (SGC-7901/CD- DP) was established in vitro. MTT assay was used to e- valuate the viability of SGC-7901/CDDP cells in vitro. Real-time PCR was used to analyse the expression of Bel-2 and Bax gene. Results MTT assay showed that oleanolie acid dramatically inhibited the proliferation of SGC-7901/CDDP cells in vitro, more than 62% SGC- 7901/CDDP cells were inhibited when the cells were treated with 100 μmol · L^-1 oleanolic acid for 72 h; Real-time PCR showed that Bax (pro-apoptotic gene) was upregulated and Bcl-2 (anti-apoptotic)was downregulated after being treated with oleanolic acid. Conclusions These results suggest that oleanolic acid inhibits proliferation of SGC-7901/CDDP cells in vitro, and its potential mechanisms may be through the upregulation of Bax and the downregulation of Bcl-2.
出处 《中国药理学通报》 CAS CSCD 北大核心 2009年第10期1334-1337,共4页 Chinese Pharmacological Bulletin
基金 黑龙江省教育厅资助项目(No11531439)
关键词 齐墩果酸 胃癌 SGC-7901 顺铂耐药 BCL-2 BAX oleanolic acid gastric cancer SGC-7901 cisplatin-resistance Bcl-2 Bax
  • 相关文献

参考文献15

  • 1李玉升.晚期胃癌的化疗与用药[J].中华医学杂志,2004,84(24):2139-2141. 被引量:13
  • 2Kerr J F,Wyllie A H,Currie A R.Apoptosis:a basic biological phenomenon with wide-ranging implications in tissue kinetics[J].Br J Cancer,1972,26(4):239-57.
  • 3LiJ,GuoWJ,等.Effects of ursolic acid and oleanolic acid on human colon carcinoma cell line HCT15[J].World Journal of Gastroenterology,2002,8(3):493-495. 被引量:80
  • 4黄炜,黄济群,张东方,张瑞玲,廖兆全.五环三萜类化合物抗人肺癌细胞侵袭和诱导细胞凋亡的研究[J].中国肺癌杂志,2003,6(4):254-257. 被引量:75
  • 5Choi C Y,You H J,Jeong H G.Nitric oxide and tumor necrosis factor-alpha production by oleanolic acid via nuclear factor-kappaB activation in macrophages[J].Biochem Biophys Res Commun,2001,288(1):49-55.
  • 6潘敏,刘纯伦,方晓杰,石建华.darbufelone对胃癌SGC-7901细胞增殖、凋亡的影响[J].中国药理学通报,2009,25(4):523-527. 被引量:7
  • 7Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2[-Delta Delta C(t)]method[J].Methods,2001,25(4):402-8.
  • 8Lacueva J,Perez-Ramos M,Soto J L,et al.Multidrug resistance-associated protein (MRP1) gene is strongly expressed in gastric carcinomas.Analysis by immunohistochemistry and real-time quantitative RT-PCR[J].Histopathology,2005,46(4):389-95.
  • 9Sarela A I,Yelluri S.Leeds upper gastrointestinal cancer multidisciplinary team.Gastric adenocarcinoma with distant metastasis:is gastrectomy necessary[J].Arch Surg,2007,142(2):143-9.
  • 10Nakamura M,Nakatani K,Uzawa K,et al.Establishment and characterization of a cisplatin-resistant oral squamous cell carcinoma cell line,H-1R[J].Oncol Rep,2005,14(5):1281-6.

二级参考文献45

共引文献173

同被引文献324

引证文献25

二级引证文献164

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部