摘要
目的观察不同月龄自发性糖尿病大鼠(GK大鼠)及链脲佐菌素(STZ)糖尿病大鼠的心肌细胞及微血管基膜厚度及Ⅳ型胶原蛋白表达程度的变化,探索一种操作简单、能够评价心肌基膜厚度的方法。方法取4、5、6、7monGK大鼠,5mon病程的链脲佐菌素糖尿病大鼠(STZ),正常7monWister大鼠各8只,对心肌切片进行六胺银染色和Ⅳ型胶原蛋白免疫组化染色。选取左心室内膜作为检测视野,通过计算机图像分析,在相同背底倍体下计算基膜阳性染色面密度、Ⅳ型胶原阳性染色面密度在所有心肌组织中的比值,作为评价心肌基膜厚度和Ⅳ型胶原表达程度的指标。结果两种糖尿病大鼠血糖、基膜阳性面密度比值(BMPSD)、Ⅳ型胶原阳性面密度比值(CPSD)均比正常大鼠增高,差异有显著性(P<0.01)。GK大鼠基膜阳性面密度比值在7mon,Ⅳ型胶原阳性面密度比值在第5月,均比4mon明显增强(P<0.01),其余各组之间有增高趋势(P>0.05)。结论对心肌切片进行六胺银染色,通过计算机图像分析,计算基膜阳性面密度比值,可以作为评价心肌基膜厚度的指标。
Aim To observe the thickness changes of cardiomyocyte and microvascular basement membranes （BM） and the expression of collagen Ⅳ in Gk rats of different months and STZ- induced diabetic rats, and to explore the method that operated easily and could assess the thickness of myocardial BM. Methods GK rats of the fourth, fifth, sixth, seventh months, Streptozotocin induced diabetes rats of fifth months and normal Wister rats were respectively divided into different groups with ev eight rats in each group. The myocardial section was stained by methenamine silver and immunohistochemistry was performed for collagen Ⅳ. Left ventricular endocardium was selected as measuring view. Through computer image analysis, the ratio of BM positive staining density（BMPSD）and positive staining density of colla-gen Ⅳ （CPSD） were calculated separately in all myocardium. Take the ratio to assess the thickness of myocardial BM and the expression of collagen Ⅳ. Results The glycemia, BMPSD and CPSD of the two diabetes rats were higher than those of the normal rats,having highly remarkable difference（P 〈 0. 01 ）. In GK rats,the BMPSD ratio in seventh months and the CPSD ratio in fifth months were significantly increased （ P 〈 0. 01 ）. Increasing tendency was observed in other groups （ P 〉 0. 05 ）. Conclusion By methenamine silver staining for myocardial section, calculating BMPSD through computer image analysis,can be an index to assess the thickness of myocardial BM.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第10期1389-1391,共3页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30500689)
山东省优秀中青年科学家基金(博士基金)资助项目(No2005BSB02023)
