摘要
目的:研究西归中总氨基酸含量的测定方法。方法:采用单因素试验对提取条件进行选择,采用分光光度法测定西归中总氨基酸含量,茚三酮显色后检测波长为570nm。结果:总氨基酸的最佳提取条件为物料比1∶15,乙醇浓度为70%,水浴回流提取2次,每次2h;标准曲线方程A=0.031 3C+0.038 9,r=0.999 7,酪氨酸含量在2～12μg/ml范围内线性关系良好。平均回收率为99.44%,RSD为2.47%(n=9)。结论:此方法简便,准确性好,为西归药材的质量控制提供了一个定量方法和参考依据。
Objective： To study the method of determination of total amino acid in seselopsis. Methods： Adopting one factor trial to choose the extract condition; after detection, using Absorption Spetrometry to determine the content of total amino acids at 570.00 nm with ninhydrin as developer. Results： The optimum condition to extract total amino acids is Seselopsis： alcohol=1：15,70% for alcohol; two bath reflux extractions for two hours; the standard curve equation of A=0.0313C＋0.0389,r=0.9997,and a good linear correlation was found in range of 2 to 12ug/ml within the tyrosine concentration. The average recovery was 99.44%, （ RSD =2.47% ,n =9 ）. Conclusion： The method is simple, accurate, which provides quantitative method and reference for quality control of seselopsis..
基金
大理学院大学生科研基金资助项目(2008DXS006)
关键词
西归
总氨基酸
分光光度法
含量测定
Seselopsis
total amino acid
absorption spectrometry
determination
