摘要
用鸭源WF01D株新城疫病毒接种对9-10日龄SPF鸡胚尿囊腔,成功增殖了该病毒,采用一步法RT-PCR技术扩增WF01D病毒的HN基因,获得了1条约1.8kb的特异性条带。PCR产物回收纯化后测序。测序结果表明,扩增片段大小为1844bp,含有1个1716bp的开放性阅读框,编码571个氨基酸。核苷酸同源性分析表明：WF01D与国内外其他NDV HN基因的同源性为81.2%-95.0%,其中与国内标准强毒株F48E9的同源性为84.3%,说明WF01D与国内外的传统毒株有较大变异。与Taiwan95株和NL/96株的同源性为93.8%和95.0%,说明WF01D与Taiwan95株和NL/96株亲缘关系较近,具有较高的相似性。
The duck NDV of WF01D strain was grown in 10 - day - old SPF embrocated eggs. The HN gene of WF01D was amplified by one step RT - PCR. The sequencing analysis showed that the sequence of the HN gene was 1844bp encoding a protein of 571 amino acids. The homology between WF01D and the NDV strains isolated inside and outside China was from 81.2% to 95.0%. The WF01D HN geneg nucleotide sequence shared 84.3% homology with that of the NDV strain F48E9. The ORF of WF01D HN gene shared 93.8% and 95.0% nucleotide homology with that of the NDV strain Taiwan95 and NL/96 respectively. The results indicated that the mutation on the HN gene had varied largely, compared with classic NDV.
出处
《安徽科技学院学报》
2009年第5期7-10,共4页
Journal of Anhui Science and Technology University
基金
国家自然科学基金项目(30671556)
安徽省教育厅自然科学基金项目(KJ2007B298)
