摘要
目的评价亚临床甲状腺功能减退症(subclinical hypothyroidism,SCH)患者血浆对氧磷酯酶1(paraoxonase1,PON1)活性及氧化应激状态,以及PON1与氧化应激的关系。方法测定56例亚临床甲减患者与48例年龄相匹配的健康体检者血浆PON1活性、血脂水平、氧化型低密度脂蛋白(ox-LDL)含量、丙二醛(MDA)水平及超氧化物歧化酶(SOD)活力,并进行相关性分析。结果亚临床甲减患者血浆PON1[(142±56)kU/L]和SOD[(83.2±26.1)U/ml]明显低于对照组PON1[(167±60)kU/L,P<0.01]和SOD[(93.1±28.2)U/ml,P<0.05];MDA[(14.10±3.01)μmol/L]和ox-LDL[(596.2±68.1)μg/L]的水平明显高于健康对照组MDA[(10.25±3.34)μmol/L,P<0.01]和ox-LDL[(467.2±60.1)μg/L,P<0.01]。亚临床甲减患者血浆PON1活性与SOD呈显著正相关(r=0.302,P<0.05),与ox-LDL(r=-0.398,P<0.01)及MDA(r=-0.401,P<0.01)呈显著负相关。结论亚临床甲减患者活性氧(ROS)产生的增加导致氧化环境的改变,可能使血浆PON1活性显著降低。PON1可能通过降低抗氧化能力机制参与了亚临床甲状腺功能减退症的病理、生理过程。
Objective To evaluate the activity of antioxidant paraoxonase 1 （ PON1） and the oxidative stress status in patients with subclinical hypothyroidism （ SCH） ,and the relationship between PON1 activity and oxidative stress parameters. Methods The fasting plasma levels of free triiodothyronine （ FT3） ,free thyroxine （ FT4） ,thyroid stimulating hormone （ TSH） ,PON1 activity,superoxide dismutase （ SOD） and malondialdehyde （ MDA） ,oxidized low-density lipoprotein （ ox-LDL） and lipids in 56 subclinical hypothyroidism patients and 48 matched normal controls were determined respectively. The correlation analysis was made between these variables. Results Compared with control,subclinical hypothyroidism had significantly lower PON1 [（ 142 ± 56） kU/L vs （ 167 ±60） kU/L]（ P〈0. 01） ,and SOD activity [（ 83. 2 ±26. 1） U/ml vs （ 93. 1 ±28. 2） U/ml] （ P〈0.05） ,and significantly higher MDA [（ 14. 10 ± 3. 01） μmol/L vs （ 10. 25 ± 3. 34） μmol/L]（ P〈0. 01） ,and ox-LDL level [（ 596. 2 ± 68. 1） μg/L vs （ 467. 2 ± 60. 1） μg/L]（ P〈0. 01） . Linear correlation analysis showed PON1 was positively correlated with SOD （ r = 0. 30,P〈0. 05） ,and negatively correlated with ox-LDL （ r = 0. 398,P〈0. 01） ,MDA （ r = 0. 40,P〈0. 01） in subclinical hypothyroidism. Conclusion Plasma PON1 activity is significantly decreased in patients with subclinical hypothyroidism. Increased reactive oxygen species （ ROS） levels in these patients may result in a pro-oxidation environment,which in turn leads to decrease PON1 activity. PON1 may participate in the pathophysiologic process of subclinical hypothyroidism through reducing antioxidant properties.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第21期2106-2108,共3页
Journal of Third Military Medical University
