摘要
目的探讨商品化的真菌药物敏感性检测方法ATB-FUNGUS3(ATB-F3法)及ROSCO纸片扩散法(ROSCO法)与标准的CLSI M27-A2(标准MIC法)方法的符合率。方法分别用法国生物梅里埃公司的ATB-F3法和丹麦ROSCO法及标准MIC法平行检测5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、伏立康唑5种抗真菌药物对280株临床分离的假丝酵母菌的体外药物敏感性并进行比较。结果ATB-F3法与标准MIC法检测5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、伏立康唑5种抗真菌药物的符合率分别为100.0%、100.0%、91.8%、91.4%、91.4%;ROSCO法与标准MIC法的符合率分别为92.9%、98.6%、88.2%、88.9%、88.6%;ATB-F3法与ROSCO法的符合率分别92.9%、98.6%、93.2%、86.8%、89.6%。结论ATB-F3法及ROSCO法与MIC法均有很高的符合率,而以ATB-F3法更高;两种商品化的方法间符合率较好,临床实验室均可选用,但宜选用MIC法或ATB-F3法以尽可能减少误差。
OBJECTIVE To compare with the coincidence rate between the mierobroth ATB-FUNGUS 3 （ATR-F3） method and ROSCO disk diffusion testing method for in vitro determination of the susceptibility of Candida spp, considering CLSI M27-A2 as the reference method. METHODS ATB-F 3 method and Rosco disk diffusion testing method and CLSI M27-A2 method were applied parallel in detecting the susceptibility of 280 strains of Candida to 5 kinds of antifungal agents and the results were compared. RESULTS The coincidence rate between ATB-F 3 method and CLSI M27-A2 method for antifungal susceptibility testing to 5-flucytosine, amphotericin B, fluconazole, itraconazole and voriconazole was 100.0%, 100.0%, 91.8%, 91.4%, and 91.4%, respectively. Between Rosco disk diffusion testing method and CLSI M27-A2 method, the coincidence rate was 92. 9%, 98.6%, 88.2%, 88.9%, and 88.6%, respectively. Between ATB-FUNGUS 3 method and Rosco disk diffusion testing method, the coincidence rate was 92. 9%, 98. 6%, 93. 2%, 86. 8%, and 89. 6%, respectively. CONCLUSIONS The coincidence is good between ATB-F3 method and ROSCO disk diffusion testing method and CLSI M27-A2 method, but ATB-F 3 is better than ROSCO. We recommend ATB-F 3 method for clinical laboratories to avoid the error.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第22期3024-3026,共3页
Chinese Journal of Nosocomiology
基金
浙江省科技厅攻关计划项目(2006C33014)
浙江省医学重点学科建设基金(07-010)
关键词
抗真菌药物
药物敏感试验
MIC法
纸片扩散法
Antifungal agent
Antifungal susceptibility test
MIC method
Disk diffusion testing
