摘要
[目的]为解决我国北方冬季污水处理困难的问题提供技术支持。[方法]运用TTC法分析了pH值、温度和时间对从低温生物膜中分离纯化鉴定得到8株耐冷菌中脱氢酶活性的影响。[结果]菌株S1、S4、S5、S7和S8在最适温度和4℃下的最适pH值都是7.5,其他3株菌在2种温度下的最适pH值不同,但都在7.5～8.5的范围内。菌株S1、S3、S4、S5和S6的最适反应温度为30℃,菌株S2、S7和S8的最适反应温度为20℃。除菌株S3外,其余菌株在4℃下的最适反应时间比最适反应温度下的长。在4℃下,菌株S3、S4和S6的最适pH值和最适反应时间分别为8.5和0.6h、7.5和12.0h、8.0和0.5h,其最高脱氢酶活性分别为7.19、6.73和7.70mg(TF)/g(SS)。[结论]菌株S3、S4和S6的脱氢酶活性较高,适合用于处理低温污水。
[ Objective ] The aim was to supply technical support for solving the problem that the sewage was difficult to dispose in north China in winter. [ Method ] The influences of pH value, temperature and time on the dehydrogenase activity in 8 strains of cold-resistant bacteria isolated, purified and identified from low-temperature biofilm were analyzed by TTC method. [ Result] The optimum pH value of the strains S1, S4, S5, S7 and S8 at both optimum temperature and 4 ℃ was 7.5 and that of the other 3 strains at the 2 temperatures were different, but they were in the range of 7.5 - 8.5. The optimum reaction temperature of the strains S1, S3, S4, S and $6 was 30 ℃ and that of the strains S2, S7 and S8 was 20 ℃. Except for the strain $3, the optimum reaction time of the other strains at 4 ℃ was longer than their optimum reaction time at optimum reaction temperature. At 4 ℃, the optimum pH values of the strains S3, S4 and S6 were 8.5, 7.5 and 8.0 resp. , their opti- mum reaction time were 0.6, 12.0 and 0.5 h resp. and their highest dehydrogenase activities were 7.19, 6.73 and 7.70 mg（TF）/g （SS） resp. [ Conclusion ] The dehydrogenase activities of the strains S3, S and S6 were higher and they were suitable to be used in processing lowtemperature sewage.
出处
《安徽农业科学》
CAS
北大核心
2009年第32期15661-15664,15700,共5页
Journal of Anhui Agricultural Sciences
关键词
低温生物膜
TTC-脱氢酶
耐冷菌
微生物活性
Low-temperature biofilm
TTC-dehydrogenase
Cold-resistant bacteria
Microbial activity
