摘要
目的探讨沙土鼠15min前脑缺血再灌注后慢性期认知障碍及组织形态学改变。方法采用双侧颈总动脉结扎法制作15min前脑缺血再灌注模型,利用"Y"字穿梭箱观察缺血4周后的记忆能力(长期记忆)及再学习能力(短期记忆),同时对脑组织进行组织学观察。结果15min前脑缺血沙土鼠,慢性期出现明显的学习记忆功能障碍,并观察到包括整个海马及躯体感觉皮层区域在内的神经细胞损伤。结论沙土鼠15min脑缺血可造成严重的组织学损害并导致慢性期学习记忆障碍。这种模型将有助于血管性痴呆症的研究。
Objective To observe the effects disruption of cognition and morphological changes on chronic phase in gerbil with 15 min forebrain ischemia reperfusion injury. Methods 15 min of forehrain ischemia reperfusion was produced in gerbils by temporarily obstructing bilateral common carotid arteries. The memory （long term memory） and relearning （impermanent memory） were measured using ＂Y＂ shuttle box in the 4th weeks following surgery. The morphological changes were observed under light microscopy. Results The ability of learning-memory were significant decreased on chronic phase in gerbil with 15 min forebrain ischemia reperfu- sion injury. Hippocampus and somatosensory cortex were damaged seriously. Conclusion Gerbils with 15 min forebrain ischemia can lead to severity of histological damage, and then lesion the ability of learning-memory on chronic phase. This model will contribute to the investigation of vascular dementia.
出处
《中国实用医药》
2009年第33期12-14,共3页
China Practical Medicine
关键词
前脑缺血
认知障碍
沙土鼠
Forebrain ischemia
Disruption of cognition
Gerbils
