摘要
采用市售次黄嘌呤核苷酸二钠盐为原料,经重结晶纯化后研制呈味核苷酸标准物质,用红外光谱法和质谱法对纯化后的次黄嘌呤核苷酸二钠盐样品进行定性鉴定,用高效液相色谱法对纯化的核苷酸进行定量分析。探讨了高效液相色谱法分析次黄嘌呤核苷酸二钠盐的分离和检测条件。试验发现有机溶剂可以显著改善次黄嘌呤核苷酸二钠盐的出峰时间,酸度和盐离子浓度可以显著改善色谱的分离度。采用多家实验室联合分析的方法对标准物质进行定值,研制的次黄嘌呤核苷酸二钠盐标准物质的纯度为99.75%,定值结果不确定度为0.32%(k=2)。
The reference material, flavor nucleotides, has been prepared through the reerystaUization and purification of commercially available disodium inosinate, which was characterized by Mass spectrometry and infrared spectroscopy. The purity was determined by high performance liquid chromatography （HPLC） and the separation and detection conditions were studied. It was found that the organic solvent can improve the retention time of disodium inosinate and the acidity and ionic concentration can improve the separating size of ehromatogram. The homogeneity and stability of the reference material were also described. Its purity and the expanded combined uncertainty were 99.75% and 0.32%（k=2） respectively.
出处
《中国测试》
CAS
2009年第6期74-77,共4页
China Measurement & Test
基金
科技部国家质检公益专项(AHY0709)
科技部科技支撑计划项目(AZC0710-2)
