摘要
建立了3位和4位联苯甲酸同分异构体混合物的高效液相色谱法（HPLC）含量测定．色谱柱为SymmetryC18，检测波长254nm，流动相为甲醇-磷酸二氢钾（1．2mol／L），流速1mL／min．3-联苯甲酸的线性范围是48-720mg／L，4～联苯甲酸线性范围是21～685mg／L；最低检出质量浓度均为0．01mg／L，相关系数分别为0．9992和0．9998；平均回收率分别是99．4％和98．8％（n=6），RSD分别为0．87％和0．93％．
A HPLC method for determination of the isomeric compounds of 3-diphenyl methyl acid and 4- diphenyl methyl acid is established. The analytical column is Symmetry C18 （150 mm × 4.6 mm, 5 μm）. The mobile phase consists of a mixture of methanol and 1.2 mol/L potassium dihydrogen phosphate with a flow rate of 1.0 mL/min. The detection wavelength is 254 nm. The standard curve of 3-diphenyl methyl acid and 4-diphenyl methyl acid are linear in the range of 48-720 mg/L and 21 -685 mg/L（ R = 0. 999 2 and R = 0. 999 8） respectively. The two lowest limits of the detections are 0.01 mg/L. The average recoveries are 99.4 % and 98.8 % （n =6） respectively. The RSD are 0.87 % and 0.93 % respectively.
出处
《河北师范大学学报（自然科学版）》
CAS
北大核心
2009年第6期770-772,共3页
Journal of Hebei Normal University：Natural Science
基金
国家自然科学基金(20575005)
