摘要
目的:观察Exendin-4对2型糖尿病大鼠肾组织基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制物-1(TIMP-1)表达的影响.方法:采用高糖高脂饮食结合小剂量链脲佐菌素(STZ)腹腔注射的方法,建立2型糖尿病大鼠模型.将成模大鼠随机分为糖尿病组,exendin-4低、中、高剂量组,并设立正常对照组.给药6wk后,采用免疫组化方法检测各组大鼠肾组织中MMP-9,TIMP-1的蛋白表达.结果:糖尿病组MMP-9,TIMP-1的表达较正常对照组明显增强(P<0.01);exendin-4各组较糖尿病组MMP-9,TIMP-1表达减弱(P均<0.01).结论:Exendin-4对2型糖尿病大鼠有一定的肾脏保护作用,其机制可能部分是通过下调2型糖尿病大鼠肾组织MMP-9和TIMP-1的表达来实现的.
AIM：To observe the effects of exendin-4 on the expression of matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinase-1（TIMP-1） in the renal tissue of type 2 diabetic rats. METHODS：The rat model of type 2 diabetes mellitus was set up by a combination of high fat diat and low dose streptozotocin（STZ）. The diabetic rats were randomly divided into 4 groups ：diabetes mellitus group（DM group）,low dose of exendin-4 group（EL group）,middle dose of exendin-4 group（EM group）,high dose of exendin-4 group（EH group）. Normal control group （NC group） was also established. After 6 weeks, the expression of MMP-9 and TIMP-1 in renal tissue was measured by immunohistochemistry. RESULTS： The expression of MMP-9 and TIMP-1 in DM group was higher than that in NC group（P 〈0.01 ） , while the expression of MMP-9 and TIMP-1 in all doses of exendin-4 groups was lower than that in DM group（ P 〈0.01 ）. CONCLUSION： Exendin-4 has some renoprotective effect on type 2 diabetic rats, partly through down-regulating the expression of MMP-9 and TIMP-1.
出处
《第四军医大学学报》
北大核心
2009年第21期2306-2309,共4页
Journal of the Fourth Military Medical University
