摘要
环介导恒温扩增技术（LAMP）是一种新型核酸扩增技术,是依赖于靶目标上6个区域的4条引物和一种具有链置换活性的DNA聚合酶Bst的作用,在60℃-65℃恒温条件下1 h之内即可完成的基因扩增技术。该技术虽然起步较晚,但发展迅速,目前在很多领域的研究和应用已经取得很大进展。论文从技术原理、引物设计、反应机制和特点等方面了介绍了环介导恒温扩增技术,并就该技术在动物细菌性病原微生物、病毒和寄生虫检测中的应用做了介绍。
Loop-mediated isothermal amplification（LAMP） is a newly developed nucleic acid amplification technique,which depends on 4 primers located in 6 separate areas of target object and Bst DNA polymerase with band replacing activity,and the target gene amplification can be finished at constant temperature of 60 ℃-65 ℃ within 1 hour.LAMP technique is developing very fast,and many great achievements in study and application have been fulfilled in various fields.The basic principle,primer design,reaction mechanism and characteristics of LAMP were intreduced,and the application of this technique in animal bacteriology,animal virology and animal parasitology were also introduced in this paper.
出处
《动物医学进展》
CSCD
北大核心
2009年第11期114-117,共4页
Progress In Veterinary Medicine
关键词
环介导恒温扩增技术
病原
检测
loop-mediated isothermal amplification（LAMP）
pathogen
detection
