摘要
[目的]探讨同时负载BMP、VEGF的PDLLA材料的体内成骨能力,以得到一种较好的可吸收骨构建材料。[方法]成骨细胞分离培养后负载到PDLLA材料中进行兔桡骨缺损修复实验,术后4、8、12周行X线、组织学及扫描电镜观察骨生成状况,12周行生物力学测试(三点折弯强度)。[结果]术后实验组各观察阶段骨形成明显高于对照组,第12周三点折弯强度与对照组相比差异有显著性(P<0.01)。[结论]负载BMP及VEGF两种细胞因子PDLLA支架材料修复兔桡骨缺损效果明显优于负载单一细胞因子PDLLA支架材料,是一种有临床应用前景的骨移植材料。
[Objective] To investigate the efect on bone formation of PDLLA （Poly-DL-Lactide） calcium BMP and VEGF and try to find the mechanism of optimizing composites so as to develop a new kind of absorbable osteosynthesis material. [ Method] High-porosity foams of PDLLA were implanted into bone defects. The specimens were harvested at 4, 8, 12 weeks after operation. The samples were examined by X-ray, histology and scanning electronic microscope （SEM） to evaluate the effectiveness of defect repair. At 12 weeks after implantation of grafts, the biomechanical testing （bending strength） was used to study the quality of bone formation. [ Result]There were significant difrences in bending strength between the experiment group and control group （P 〈0. 01 ）. The experiment group was signficantly higher in bone formation compared with the control groups in every stage of postimplantation （ P 〈 0.01 ）. [ Conclusion ] The PDLLA calcium with both BMP and VEGF composites have better osteoconductibility than calcium with novel bone formation. A composite of porous PDLLA Calcium BMP and VEGF is a new valuable bone transplantation material.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2009年第22期1735-1738,共4页
Orthopedic Journal of China
关键词
超聚消旋乳酸
骨形态发生蛋白
血管内皮生长因子
骨缺损
骨髓基质干细胞
poly-DL-Lactide
bone morphogenetic protein
vascular endothelial growth factor
bone defects
marrow mesenchymal stem cells
