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高浓度氧对新生大鼠肺血管内皮生长因子及其受体表达的影响 被引量：10

Effects of prolonged exposure of high concentration of oxygen on expression of vascular endothelial growth factor and its receptors in neonatal rat lungs
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摘要 目的研究持续吸入75%氧对新生大鼠肺血管内皮生长因子(vascular endothelial growth factor,VEGF)及其受体(VEGFR1和VEGFR2)表达的影响,探讨较高浓度吸氧对肺血管发育的影响及与支气管肺发育不良(bronchopulmonary dysplasia,BPD)的关系。方法新生足月Sprague-Dawley大鼠48只,随机分为对照组和实验组。实验组生后12h开始持续吸入75%氧气。分别于实验开始后的7d、14d和21d处死留取肺组织标本,苏木精-伊红染色观察病理改变,免疫组化检测VEGF及其受体蛋白表达,RT-PCR检测其mRNA表达。结果实验组大鼠肺组织结构发生类似"新型"BPD的病理改变。75%氧暴露21d时,VEGF(10.9±2.7vs30.8±6.4)、VEGFR1(5.4±1.4vs15.6±3.4)和VEGFR2(11.3±2.6vs21.7±4.5)的蛋白表达均较对照组减少(P<0.05);VEGF(1.6vs3.3)、VEGFR1(0.4vs6.6)及VEGFR2(0.5vs4.9)的mRNA表达均较对照组减少(P<0.05)。结论在新生大鼠中,长时间吸入较高浓度氧可能通过抑制肺血管发育导致BPD的发生。 Objective To study the effects of prolonged 75% oxygen exposure on the expression of vascular endothelial growth factor （VEGF） and its receptors （VEGFR1 and VEGFR2 ） in the neonatal rat lungs and to elucidate the effects of prolonged exposure of high concentration of oxygen on lung vascular development and its relationship with bronchopulmonary dysplasia （BPD）. Methods Forty-eight Sprague-Dawley rat pups were randomly exposed to air （ control group） and 75% oxygen （ experimental group） 12 hrs after birth. The rats were sacrificed 7, 14 and 21 days after exposure and their lungs were sampled. The lung sections were stained with hematoxylin and eosin for histological evaluation. Expression of VEGF, VEGFR1 and VEGFR2 protein and mRNA was detected by immunohistochemistry and RT-PCR. Results After being exposed to 75% oxygen for 21 days, lung tissues had pathological changes as ＂new＂ BPD. Expressions of VEGF protein （ 10.9± 2.7 vs 30.8± 6.4）, VEGFR1 protein （ 5.4± 1.4 vs 15.6±3.4） and VEGFR2 protein （ 11.3 ± 2.6 vs 21.7± 4.5 ） on day 21 in the experimental group decreased significantly as compared with the control group （ P 〈 0.05 ）. The expression of VEGF mRNA （ 1.6 vs 3.3）, VEGFR1 mRNA （0.4 vs 6.6） and VEGFR2 mRNA （0.5 vs 4.9） on day 21 in the experimental group also decreased significantly as compared with the control group （ P 〈 0.05 ）. Conclusions Prolonged exposure of high concentration of oxygen may cause BPD possibly by inhibiting lung vascular development in neonatal rats.
作者 冯海燕 陆爱珍 张晓波 王立波 陈超
机构地区 复旦大学附属儿科医院
出处 《中国当代儿科杂志》 CAS CSCD 北大核心 2009年第11期927-930,共4页 Chinese Journal of Contemporary Pediatrics
关键词 血管内皮生长因子 受体 血管内皮生长因子 高浓度氧 新生大鼠 Vascular endothelial growth factor Receptors, vascular endothelial growth factor High concentration of oxygen Neonatal rats
分类号 R722.1 [医药卫生—儿科]
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参考文献10

二级参考文献18

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共引文献16

同被引文献167

  • 1王伟,韦伟,王安茹,蒙晶,宁琴,罗小平.6O％氧暴露对早产大鼠肺血管内皮生长因子及其受体表达的影响[J].中华围产医学杂志,2007,10(3):186-189. 被引量：3
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引证文献10

二级引证文献30

中国当代儿科杂志
2009年 第11期

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