摘要
根据罗非鱼微卫星遗传图谱,在不同的连锁群上随机选择30个微卫星位点,研究它们在192尾不同家系吉富罗非鱼(Genetically Improved Farmed Tilapia,GIFT)中的遗传多样性及这些位点与体质量、体形性状的相关性。结果表明,30个位点共检测出126个等位基因,片段长度124～310bp。各位点等位基因数为1～7个,平均等位基因数4.2个;多态信息含量(PIC)为0.2594～0.7167,平均0.5808;杂合度(H)为0.3064～0.7531,平均0.6322,表明该吉富罗非鱼群体遗传多样性比较丰富。最小二乘方差分析结果显示,位点GM041、GM542和GM304与吉富罗非鱼雌鱼体质量相关;位点GM041、UNH860、GM519和GM304与雄鱼体质量相关,位点UNH952与雄鱼体形相关。位点GM041、UNH860、GM304的AA基因型和位点GM519的DE基因型都有可能作为以体质量为选育目标的辅助标记,其中UNH860和GM304的AA基因型有望成为作为以体质量为选育目标的首选辅助标记。实验结果说明,吉富罗非鱼具有与体质量相关的标记越多,体质量也越大。
In this study,30 microsatellite loci were chosen randomly from different linkage groups according to the microsatellite genetic map of tilapia,and its polymorphisms and association of the DNA markers with body weight and body shape were researched in 192 GIFT（genetically improved farmed Tilapia）distributed in different families. The results showed that a total of 126 alleles were found through 30 loci,and the DNA fragment length of these alleles ranged from 124-310 bp.The number of alleles per locus was 1-7,with an average of 4.2,the polymorphism information content（PIC）was 0.259 4-0.716 7,with an average of 0.580 8,while the heterozygosity（H）was 0.306 4-0.753 1,with an average of 0.632 2.The data above indicated that the genetic diversity of the GIFT population was in high level.The least-square method was used to analyse the microsatellite markers associated with the traits of body weight and body shape.The results suggested that GM041,GM542 and GM304 in female,GM041,UNH860,GM519 and GM304 in male were associated with body weight,and UNH952 in male was associated with body shape. Genotype AA of GM041,UNH860,GM304 and genotype DE of UNH519 might be regarded as the candidate-marker for body weight trait,among which genotype AA of UNH860 and GM304 were expected to be the preferred marker.The results also showed that the more body associated markers,the larger body weight in GIFT.
出处
《中国水产科学》
CAS
CSCD
北大核心
2009年第6期824-832,共9页
Journal of Fishery Sciences of China
基金
国家科技基础条件平台资助项目(2007DKA30470_003)
中国水产科学研究院基本科研业务费专项资金(2009JBFC02
2009JBFB05)
