摘要
目的探讨弓形虫感染对大鼠大脑组织神经丝（NF）mRNA表达和细胞免疫水平的影响。方法将4周龄雄性SD大鼠分成两组（每组10只）：弓形虫感染组腹腔感染2×10^10个／L弓形虫速殖子悬液2ml：对照组腹腔注射灭菌生理盐水2ml。9周后，应用RT-PCR法检测大鼠大脑组织中高相对分子质量NF（NF-H）、中相对分子质量NF（NF—M）和低相对分子质量NF（NF—L）mRNA表达水平；流式细胞术检测大鼠外周血CD3^＋、CD4^＋、CD8^＋T淋巴细胞；酶联免疫吸附试验测定其血清γ干扰素（IFN-γ）、肿瘤坏死因子α（TNF-α）、白细胞介素4（IL-4）水平。结果大鼠弓形虫感染9周后，大脑组织NF—L、NF—HmRNA表达量（0．51±0．06、0．68±0．05）较对照组（0．79±0．03、0．76±0．05）明显下降（t值分别为3．41、2．17，P〈0．01或〈0．05）：NF—MmRNA表达量（0．69±0．02）与对照组（0．72±0．03）比较，差异无统计学意义（t=2．09，P〉0．05）。弓形虫感染组大鼠的CD4^＋T细胞[（32．19±5．01）％]和CD8^＋T细胞[（20．06±2．28）％1与对照组[（35．82±3．71）％、（22．06±3．90）％]比较，差异均无统计学意义（t值分别为1．69、1．88，P均〉0．05）。弓形虫感染组大鼠血清IFN-γ[（6．03±0．16）ng／L]、TNF-α[（18．05±1．93）ng／L]、IL-4[（15．76±1．59）ng／L]水平均高于对照组[（4．77±0．13）、（9．54±1．57）、（5．67±0．42）ng／L，t值分别为2．81、2．74、2．63，P均〈0．05]。结论弓形虫感染可导致大鼠大脑组织中NF亚单位mRNA表达水平下降。血清IFN-γ、TNF—α、IL-4水平升高。
Objective To investigate the alterations in levels of neurofilament （NF） mRNA and cellular immunity in rats infected with Toxoplasma gondii. Methods Four-week old male SD rats were randomly divided into two groups with ten animals for each. The subjects in the experimental group were i.p. injected with 2×10^10/L toxoplasma taehyzoits 2 ml for each. Meanwhile, each rat in the control group was i.p. injected with NaCl2 ml. After 9 weeks, mRNA levels of light molecular NF（NF-L）, medium molecular NF（NF-M） and high molecular NF （NF-H） in cerebrum were determined by RT-PCR. The percentages of CD3^＋、CD4^＋、CD8^＋ T lymphocytes were examined by means of flow cytometry, and the peripheral blood serum levels of IFN-γ, TNF-α, IL-4 were analyzed by ELISA. Results The mRNA levels of NF-L, NF-H in rat cerebrum（0.51 ± 0.06,0.68 ± 0.05） decreased than control group（0.79 ± 0.03,0.76 ± 0.05, t = 3.41,2.17, P〈 0.01 or 〈 0.05）, the NF-M mRNA（0.69 ± 0.02） had no statistical significance（t = 2.09, P 〉 0.05 ） than control group（0.72 ± 0.03）. No significant changes were observed on the levels of CD4^＋T lymphocytes [ （32.19 ± 5.01 ）% ] and CD8^＋ T lymphocytes [ （20.06 ± 2.28 ）% ], compare with the control group[ （35.82 ± 3.71 ）%, （22.06 ± 3.90）%, t = 1.69,1.88, all P 〉 0.05]. The serum levels of IFN-γ[（6.03 ± 0.16）ng/L], TNF-α[（18.05 ± 1.93）ng/L], IL-4[（15.76 ± 1.59）ng/L] in experimental rats were significantly higher than those in the controls[ （4.77 ± 0.13）, （9.54 ± 1.57）, （5.67 ± 0.42）ng/L, t = 2.81, 2.74,2.63, all P 〈 0.05]. Conclusion Toxoplasma gondii infection can decrease NF subunit mRNA expression levels in rat brain tissue and increase serum levels of IFN-γ,TNF-α and IL-4 .
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2009年第6期601-603,共3页
Chinese Jouranl of Endemiology
基金
江苏省卫生科研基金（X200739）
