摘要
目的对性成熟前小鼠的窦前卵泡采用不同的方法进行玻璃化冷冻,探讨不同冷冻方法对卵泡复苏率的影响。方法用Ⅰ型胶原酶和脱氧核糖核酸酶Ⅰ分离10～14dICR雌鼠的卵巢,共进行A、B两个实验,每次实验对获得的卵泡分为4组,1组作为新鲜对照组,其余3组采用不同的冷冻方法处理,试验A采用常规玻璃化法、-205℃玻璃化法和液氮网篮法;试验B采用-205℃玻璃化法、液氮网篮法和-205℃表面玻璃化法,卵泡复苏后进行台盼蓝染色,计算各组卵泡复苏率。结果液氮网篮和-205℃玻璃化冷冻的冷冻效果一致,两种方法的卵泡复苏率差别无统计学意义;常规玻璃化的冷冻效果最差,-205℃表面玻璃化冷冻的冷冻效果最好,其冷冻小鼠窦前卵泡的复苏率达(83.02±4.10)%。结论-205℃表面玻璃化是一种较好的冷冻窦前卵泡的方法。
Objective To investigate the influence of different methods on the recovery rate of preantral follicle of immature mice after they were treated with vitrification and freeze-thawing. Methods Collagenase I and DNase I were used to separate the ovarian tissue of the ICR female mice（10-14 d of age）. Two experiments （A and B） were carried out,in each of which the follicles obtained after cleaning were divided into four groups, one as a control group, the other three groups given vitrifiable cryoprotection with different methods. In Experiment A, conventional vitrification,- 205 ℃ vitrification and metal net were used, while in Experiment B, - 205 ℃ vitrification,metal net and - 205 ℃ surface vitrification adopted. After recovery, follicles were stained with trypan blue, and the recovery rates were calculated. Results In the two experiments,the effect of metal net and -205 ℃ vitrification are consistent. There were not significant differences between them. The effect of conventional vitrification was the worst. In addition,the effect of -205 ℃ surface vitrification was the best,with a recovery rate of （83.02±4. 10）%. Conclusion -205 ℃ surface vitrification is a desirable way to freeze preantral follicles.
出处
《福建医科大学学报》
2009年第5期377-380,共4页
Journal of Fujian Medical University
基金
教育部科学技术重点基金(204073)
关键词
卵泡
卵子
冷冻
组织保存
小鼠
近交ICR
ovarian follicle
ovum
freezing
tissue preservation
mice, inbred ICR
