摘要
【目的】了解AP2/EREBP家族基因参与逆境反应分子机理,为水稻抗逆相关基因的克隆及揭示水稻抗逆分子调控机理奠定基础。【方法】采用基因芯片技术分析AP2/EREBP家族基因在水稻幼苗受PEG、低温、高盐、ABA、GA等处理下的表达谱变化,并通过实时定量PCR技术对部分具有明显表达特点基因的胁迫表达谱进行验证。【结果】点制了水稻AP2/EREBP转录因子家族的基因芯片,检测到42个胁迫差异表达的AP2/EREBP基因。实时定量PCR所得结果与基因芯片结果基本吻合,说明芯片结果可靠。两个AP2/EREBP基因对所有胁迫反应相同,其它差异表达的AP2/EREBP基因对不同胁迫反应各不相同。【结论】研究发现两个AP2/EREBP基因在水稻应对外界胁迫反应中起核心分子调控作用;不同差异表达AP2/EREBP基因在水稻响应不同胁迫反应过程中具有相同或者不同的分子应答机理。
[Objective] The AP2/EREBP transcription factors are known to be unique in plants, and they are involved in growth, development and signal transduction in many physiological and biochemical responses. In this study, all the rice AP2/EREBP genes were cloned and used to dissect their expression alteration responsive to different abiotic stresses, providing a foundation for exploring the mechanism office stress-resistance molecular regulation. [Method] A microarray gene chip was used to analyze the AP2/EREBP gene expression profiling in rice seedlings under the treatment of PEG, low-temperature, high-salt, ABA and GA. The expression patterns of selected differentially expressed genes were further identified by quantitative real-time PCR. [Result] Forty-two AP2/EREBP genes were detected differentially expressed under at least one abiotic stress by using a microarray chip containing the whole AP2/EREBP gene family, and the alteration of gene expression from army data was confirmed by qRT-PCR. Two AP2FEREBP genes were found differentially regulated at the same direction by all stresses; however each of the rest forty differentially expressed AP2/EREBP genes had the same or distinct expression pattern responsive to different abiotic stresses. [Conclusion] Two AP2/EREBP genes might play key molecular regulation roles responding to abiotic stresses in rice. The identified differentially expressed AP2/EREBP genes have the same or distinct molecular responsive mechanism to different abiotic stresses.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第11期3765-3773,共9页
Scientia Agricultura Sinica
基金
国家高技术研究发展计划资助项目(2007AA10Z191)
