摘要
目的:观察苦参碱对U937细胞内周期调控蛋白的影响。方法:采用四甲基偶氮唑盐(methyl thiazolyl tetrazolium,MTT)法检测细胞生长受抑情况,流式细胞仪检测细胞周期分布,免疫细胞化学法及Western blot检测cyclin E、cyclin A蛋白表达。结果:MTT检测显示苦参碱能显著抑制U937细胞增殖,其抑制作用呈时间和剂量依赖性;U937细胞受阻于S期,苦参碱作用后细胞内cyclin E及cyclin A表达显著下调。结论:苦参碱能减少细胞内cyclin E及cyclin A蛋白的表达,从而抑制U937细胞增殖。
AIM ： To investigate the influence of matrine on cyclin E and cyclin A in U937 cell strain. METHODS ： The inhibitory effect of matrine on proliferation in U937 cell strain was observed by MTF. The distribution of cell cycle was measured by flow cytometry. Immunocytochemical method and Western blot were used to detect the protein expression of eyclin E and cyclin A. RESULTS ： After being treated with matrine, the proliferation of U937 cell strain was inhibited significantly, the inhibiting effect conformed to time and dose-dependence, cells were arrested in S-phase. The expression of cyelin E and cyclin A was down-regulated significantly in a dose-dependence manner. CONCLUSION： Matrine could reduce the expression of cyclin E and cyclin A and inhibit the proliferation in U937 cell strain.
出处
《中成药》
CAS
CSCD
北大核心
2009年第11期1658-1661,共4页
Chinese Traditional Patent Medicine
基金
重庆市卫生局科研基金课题(07-2-035)
重庆市教委科研项目(KJ080321)
重庆医科大学创新基金(XBYB2007024)
