摘要
棉纤维是研究植物细胞伸长和细胞壁建成以及纤维素生物合成的优良模型,迄今为止,已经分离了许多纤维特异/优势表达的基因。为了便于这些基因的图位克隆使其能够应用于棉花纤维品质的改良中,本研究采用分离群体定位法和Blast分析法对这些基因进行染色体定位。利用陆地棉、海岛棉BC1种间分离群体,将GhCFE定位在第6染色体,GhGLP1-250定位在第19染色体。Blast分析将11个基因定位到棉花染色体上。这些基因与棉纤维的伸长和细胞壁的合成相关,与这些基因连锁标记的获得有助于棉花纤维长度和强度的分子标记辅助选择。
Cotton is one of the most important economic crops in the world, and developing cotton fiber is a perfect experimental model for studying the mechanism of cell elongation, wall development and cellulose biosynthesis in plants. Up to now, many genes that expressed specifically or preferentially in developing cotton fiber have been isolated. In order to facilitate map-based cloning of these genes and make them useful in cotton fiber breeding, genetic mapping was performed to chromosome localization of these genes by experimental and Blast analysis. GhCFE and GhGLP1-250 were mapped to chromosome 6 and 19, respectively, in the [（Emian22 X 3-79） X Emian22] BC1 population. Eleven genes were mapped corresponding chromosomes by Blast analysis. These mapped genes are involved for cotton fiber elongation and cell wall synthesis, and markers linked to these genes will be helpful in marker assisted selection for cotton fiber length and strength.
出处
《棉花学报》
CSCD
北大核心
2009年第6期435-441,共7页
Cotton Science
基金
国家重点基础研究发展计划(863计划)项目(2006AA10Z153
20060AA00105)
