摘要
目的探讨嗜酸性粒细胞趋化因子(Eotaxin)、T淋巴细胞激活上调性表达分泌因子(RANTES)在正常妊娠免疫耐受及反复性自然流产中的作用。方法构建正常妊娠小鼠模型CBA/J×BALB/c(正常妊娠组)和反复性自然流产小鼠模型CBA/J×DBA/2J(自然流产组);采用免疫组织化学方法检测胎盘细胞趋化因子Eotaxin、RANTES的表达;采用酶联免疫吸附试验(ELISA)检测胎盘细胞培养上清液的白细胞介素(IL)-4、干扰素(IFN)-γ水平,分析胎盘细胞趋化因子Eotaxin、RANTES与细胞因子IL-4、IFN-γ水平的相关性。结果自然流产组的胚胎丢失率为18.3%,显著高于正常妊娠组的3.6%(P<0.05)。正常妊娠组的胎盘细胞Eotaxin的表达阳性率为53.6%,显著高于自然流产组的40.8%(P<0.05);而正常妊娠组的胎盘细胞RANTES的表达阳性率为44.3%,显著低于自然流产组的62.3%(P<0.05)。正常妊娠组的IL-4水平为(1.33±0.43)ng/L,显著高于自然流产组的(0.78±0.41)ng/L(P=0.002);正常妊娠组的IFN-γ水平为(1.24±0.57)ng/L,显著低于自然流产组的(1.67±0.44)ng/L(P=0.038)。自然流产组中,胎盘细胞Eotaxin的表达与IL-4水平呈正相关(r=0.752,P=0.003),RANTES的表达与IFN-γ水平亦呈正相关(r=0.658,P=0.014);正常妊娠组中,RANTES的表达与IFN-γ水平呈正相关(r=0.649,P=0.012),IL-4水平随Eotaxin表达阳性率的上升而显示出增加趋势。结论Eotaxin、RANTES在妊娠免疫耐受形成和自然流产的发病机制中可能发挥重要作用。
Objective To investigate the role of chemokine condensed from eosinophil chemotaxin （Eotaxin）, reduced upon activation, normal T cell expressed and secreted （RANTES） expression in the placenta cells in normal pregnant immune tolerance and repeated spontaneous abortion. Methods Normal pregnant mouse model CBA/J× BALB/c （NNP group） and spontaneous abortion mouse model CBA/J× DBA/2J model （SA group） were established. The expression of Eotaxin, RANTES on cells of the placenta was detected by immunohistochemistry method; the concentrations of interleukin （IL）-4, interferon （IFN）-γ in culture supernatant were determined by ELISA; and the correlation of the chemokine expression with the concentration of cytokine was analyzed. Results The embryo loss rate of SA group （ 18.3% ） was significantly higher compared with that of the NNP group （3.6%, P〈0.05）. The positive rate of Eotaxin in the NNP group （53.6%） was significantly higher than that in the SA group （40.8%, P〈0.05）, and the positive rate of RANTES in the NNP group （44.3%） was significantly lower than that in the SA group （62.3%, P〈0.05）. The concentration of IFN-γ （[1. 67 ± 0. 44] ng/L） in the SA group was significantly higher and the concentration of IL-4 （[0.78±0. 41]ng/L） was significantly lower than those in the NNP group （ vs. [ 1.24± 0. 57] ng/L and [ 1.33 ± 0. 43] ng/L, respectively, all P〈0.05）. We also found that in the SA group the positive rate of Eotaxin was positively correlated with the concentration of IL-4, and the positive rate of RANTES was positively correlated with the concentration of IFN-γ （ r = 0. 752, 0. 658, P = 0. 003, 0. 014）. in the NNP group the positive rate of RANTES was positively correlated with the concentration of IFN-γ（r = 0. 649, P= 0. 012）. IL-4 level increased with the positive rate of Eotaxin. Conclusion Eotaxin and RANTES may play an important role in the pathogenesis of pregnancy immune tolerance and spontaneous abortion. （Shanghai Med J, 2009, 32： 906-909）
出处
《上海医学》
CAS
CSCD
北大核心
2009年第10期906-909,I0002,共5页
Shanghai Medical Journal
基金
上海市自然科学基金资助项目(07ZR14072)
关键词
趋化因子
嗜酸性粒细胞趋化因子
T淋巴细胞激活上调性表达分泌因子
自然流产
Chemokine
Condensed from eosinophil chemotaxin
Reduced upon activation, normal T cell expressed and secreted
Spontaneous abortion
