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玫瑰黄链霉菌Men-myco-93-63负调控基因nsdAmgh的克隆及序列分析 被引量:4

Cloning and Sequence Analysis of nsdA_(mgh) Gene in Streptomyces roseoflavus Men-myco-93-63
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摘要 nsdA(Negative regulator of Streptomyces differentiation)基因是天蓝色链霉菌中发现的与抗生素合成相关的负调控基因。根据天蓝色链霉菌A3(2)的序列设计引物扩增玫瑰黄链霉菌Men-myco-93-63菌株中的该基因,经测序和序列分析表明,由保守序列引物nsdA-2R和nsdA-2L克隆到的Men-myco-93-63菌株中的该基因800 bp片段与已知nsdA基因核苷酸保守区域序列同源性达到99%,由全长序列扩增引物nsdA WZ-R和nsdA WZ-L克隆到的该基因包含一个完整的开放阅读框,共编码369个氨基酸,与变铅青链霉菌和天蓝色链霉菌A3(2)中的nsdA基因的核苷酸序列同源性达到100%,氨基酸序列同源性达到99%。该全长基因命名为nsdAmgh,其在玫瑰黄链霉菌Men-myco-93-63中的发现为阻断该负调控基因以期获得抗生素高产工程菌株提供了重要依据。 nsdA (Negative regulator of Streptomyces differentiation )is globally negative regulator gene first found in Streptomyces coelicolor. In this study, nsdA gene of Streptomyces roseoflavus strains Men-myco-93-63 was amplified using the primer sequences designed from. Streptomyces coelicolor A3 (2). After sequencing, Blastn, and Blastx, it showed that the cloned fragment from Streptomyces roseoflavus Men-myco-93-63 with the primer of nsdA-2R and nsdA-2L compairing with reported nsdA conservative nucleotide sequence homology on the region reached 99 %. The full-length gene sequence of Men-myco-93-63 nsdA gene amplification with primers of nsdA WZ-R and nsdA WZ-L had a complete open reading frame encoding 369 amino acids, with the Streptomyces lividans and Streptomyces coelicolor A3 (2)nsdA gene at nucleotide sequence 100%, and amino acid sequence homology up to 99% . The full-length nsdA gene of Streptomyces roseoflavus Men-myco-93-63 was named nsdAmgh, which will promote the obtaintion of high-yielding antibiotic strains of Streptomyces roseoflavus Men-myco-93-63 through interrupting the negative regulator.
出处 《华北农学报》 CSCD 北大核心 2009年第5期77-80,共4页 Acta Agriculturae Boreali-Sinica
基金 国家自然科学基金项目(30800734) 河北省自然科学基金项目(C200900598) 国家高技术研究与发展计划“863”项目(2006AA10A211)
关键词 玫瑰黄链霉菌MEN-MYCO-93-63 nsdA基因 克隆 序列分析 Streptomyces roseoflavus Men-myco-93-63 nsdA gene Cloning Sequence analysis
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  • 1余贞,王茜,邓子新,陶美凤.负调节基因nsdA在链霉菌中同源性及激活沉默抗生素合成基因簇的研究[J].生物工程学报,2006,22(5):757-762. 被引量:21
  • 2Takano E,Tao M, Long F, et al. A rare leucine codon in adpA is implicated in the morphological defect of bldA mutants of Streptomyces coelicolor [J]. Molecular Microbiology, 2006, 50:475 - 486.
  • 3Li W, Ying X, Guo Y, et al. Identification of a gene negatively affecting antibiotic production and mor-phological differentiation in Streptomyces coelicolor A3 (2)[J].Journal of Bacteriology,2006,188:8368 - 8375.
  • 4陈芬,熊伟,闵勇,范与庆,梁运祥,吕和平,邢仁昌,郑应华.肉桂地链霉菌接合转移体系的构建及nsdA基因中断对其次级代谢的影响[J].农业生物技术学报,2007,15(6):1042-1047. 被引量:11
  • 5Liu D Q. Biological control of Streptomyces scabies and other plant pathogens [ D ]. Thesis, The University of Minnesota, USA, 1992.
  • 6Liu D Q, Xiao K. Isolation methods and soil sources selecting Streptomyces spp. suppressive to S. scabies and other plant pathogens[C]. Advances in Biological Control of Plant Diseases, Beijing, 1992 : 197 - 205.
  • 7Liu D Q, Ersonna, Kinkelll. Selection an characterization of Streptomyces suppressive to the potato scab pathogens [J]. Microbiol, 1996,42:487 - 502.
  • 8刘大群,杨文香,祁碧菽,王朝花.拮抗链霉菌Men-myco-93-63及其发酵液对棉花黄萎病菌生长的影响[J].河北农业大学学报,1999,22(4):79-82. 被引量:35
  • 9张汀,赤国彤,杨文香,等.Men-myco-93-63发酵液不同剂型防治茄子黄萎病的田间小区药效研究[C]//第二届全国绿色环保农药新技术、新产品交流会论文集,2003:420-423.
  • 10Yang W X, Zhang T, Liu D Q. Biological control cucumber powdery mildew using culture flit rate of an tagonistic Streptomyces in green house [ C ]//The 2nd International Work shop on White Agriculture,2004: 18- 21.

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