摘要
经PCR扩增获得了纳豆激酶基因(NK)。将此基因插入原核表达载体pTWIN1中,使之与几丁质结合域(CBD)一内含肽(intein)融合,获得原核表达质粒pTWIN1/NK。转化宿主菌E.coliBL21(DE3),在IPTG诱导下进行纳豆激酶的表达研究。SDS-PAGE电泳分析结果表明,重组蛋白在BL21(DE3)中获得高效表达,在低温诱导时主要以可溶性蛋白的形式存在。
Nattokinase gene was amplified using polymerase chain reaction （PCR）, and cloned into prokaryotic expression vector pTWIN1. The expression plasmids of pTWIN1/NK was constructed and was further transformed into E. coli BL21 （DE3）. Expression of the enzyme was performed at different temperatures with IPTG induction. SDS - PAGE showed that nattokinase was expressed in soluble form at a low temperature. The cloning and expression of nattokinase would be helpful in producing nattokinase by gene engineering strain.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2009年第4期398-401,共4页
Journal of Jilin Agricultural University
基金
吉林省科技发展计划项目(20070579)
关键词
纳豆激酶基因
克隆
表达
nattokinase gene
cloning
expression
