摘要
目的了解常规体外授精(in-vitro fertilization,IVF)中正常受精率患者处理前后精液的参数范围及其对受精结果的影响。方法回顾性分析在本生殖医学中心接受常规IVF治疗且获得正常受精及移植胚胎的408个周期的精液参数及其与受精结果的关系。结果精子密度正常者399人次(97.79%),而活力正常者仅21人次(5.15%)。处理前精液量0.2～8ml,精子密度(3～400)×106个/ml,活率10%～85%,前向运动精子比率(progressive motile sperm,PMS%)2%～40%,前向运动精子总数(total progressive motile sperm count,TPMSC)2～597×106。处理后的精子活率、PMS%及精子总数与处理前精子密度、活率、PMS%以及A+B级精子总数均呈显著的正相关(P<0.05)。处理后的精子活率与受精率明显相关(r=0.132,P=0.008)。结论常规IVF正常受精者精液参数波动范围大,轻、中度的弱精或少精可能不影响正常受精,但处理后的精子活率可影响受精率。
Objective To investigate the semen relative parameters and their effects on feltilization outcomes. Methods Totally 408 semen samples with normal fertilization rates in conventional in vitro fertilization and embryo transfer （IVF-ET） cycles in our department from January 2006 to July 2008 were collected. The relationship of the semen parameters and fertilized outcomes were retrospectively analyzed. Results Among 408 samples, there were 399 samples （about 97.79qc ） had normal sperm density, but only 21 samples （5.15%） had normal motility. The sperm density was 3 to 400 106/ml, its motility 10% to 85%, percentage of progressive motile sperm （ PMS% ） 2% to 40%, and total progressive motile sperm count （ TPMSC ） was 2 to 597 ×10^6. The motility, PMS% and total sperm count after sperm separation were positively correlated with the density, motility, PMS% and TPMSC before separation （P 〈 0.05）. There was a positive correlation between the fertilization rate and separated sperm motility （r = 0. 132, P 〈 0.01 ） , other parameters have no effect on fertilization rate. Conclusion Semen parameters have a wild range for the patients with normal fertilization rate in eonventional IVF. Mild and moderate oligoasthenospermia maybe have no effect on fertility. However, the motility after sperm separation can affect fertilization rate.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第23期2378-2380,共3页
Journal of Third Military Medical University
关键词
常规体外受精
精液参数
精子分离
conventional in vitro fertilization
semen/sperm parameter
sperm separation
