摘要
采用荧光法测定富硒乳酸菌中有机硒含量,对测定过程中影响测定结果的各种主要因素以及最佳条件的确定进行了探讨。结果表明:该方法的检出限为0.25μg/L,加标回收率为94.6%～103.3%,相对标准偏差小于2.46%。该法具有简便、灵敏、稳定、准确等优点,适于富硒乳酸菌中有机硒的分析测定。
The content of organic selenium in Se-enriched Lactobacillus was determined by fluorometry. The main factors affecting the determination results and the optimal conditions were analyzed in detail. The results showed that the limit of detection was 2.5 μg/L, the relative standard deviation（RSD） was less than 2.46%, and the recovery 94.6% - 103.3%. With the advantages of high simplicity, sensitivity, stability and accuracy, the method was suitable for the determination of organic selenium in Se- enriched Lactobacillus.
出处
《饮料工业》
2009年第11期28-31,共4页
Beverage Industry
关键词
富硒乳酸菌
有机硒
荧光法
Se-enriched Lactobacillus
organic selenium
fluorometry
