摘要
目的:探讨机体HLA-DRB1*07,13等位基因对基因重组乙肝疫苗免疫效果的影响。方法:选取完成基因重组乙肝疫苗全程接种(10μg/次,0、1、6月)的广西籍汉族健康大学生896名,于末次接种疫苗后的第6个月采血检测血清抗-HBs水平,对无或低应答者再次接种基因重组乙肝疫苗20μg,4周后筛选出无或低应答者99名及初次检测中或强应答者136名作为研究对象,应用PCR-SSP技术对研究对象外周血HLA-DRB1*07,13等位基因进行检测。结果:HLA-DRB1*07在无或低应答组中的表达频率为16.16%,显著高于中或强应答组的表达频率(4.41%)(P<0.05);HLA-DRB1*13在无或低应答组和中或强应答组的表达频率分别为1.01%和3.68%,两组比较无统计学差异(P>0.05)。结论:基因重组乙肝疫苗无或低应答与HLA-DRB1*07基因密切相关;而HLA-DRB1*13对乙肝疫苗的免疫应答无明显影响。
Objective：To investigate the effect of HLA-DRB1 ＊ 07,13 allelic genes on the immune response to hepatitis B vaccination. Methods： 896 healthy college students of Han nationality from Guangxi province, who had received standard courses of vaccination with recombinant hepatitis B virus vaccine, were tested the level of anti-HBs with ELISA method at the sixth month after their last vaccination. The nonor hypo-responders were selected to receive another 20μg doses of recombinant vaecine and were examined anti-HBs once more four weeks later. 99 non- or hypo-responders and 136 medium or h）33er-responders were selected for the study subjects. HLA-DRB1 ＊ 07, 13 allelic genes of the subjects were detected by polymerase chain reaction using sequence specific primer （PCR-SSP） method. Results： The frequencies of HLA- DRB1 ＊ 07 gene in the non- or hypo-responders group were significantly higher than that in the medium or hyper-responder group （ 16. 16% vs 4.41%,P〈0.05）. No significant difference of HLA-DRB1 ＊ 13 gene between the two groups （1.01% vs 3.68%, P = 0.389）.Conclusion：There is a close relationship between the expression of HLA-DRBI ＊ 07 gene and the non- or hypo-response to HB vaccine; No relationship between HLA-DRBI ＊ 13 gene expression and the response to HB vaccine is found.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2009年第11期996-998,1002,共4页
Chinese Journal of Immunology
基金
广西卫生厅课题(No.Z2005062)
广西自然科学基金课题(No.桂科自0728159)资助
