摘要
目的:观察脓毒症大鼠肺血管内皮细胞(VEC)、细胞间黏附分子1和E-选择素的变化特点并探讨其意义。方法:60只SD大鼠随机分成对照组和脓毒症组。以脂多糖(LPS)静脉注射制备大鼠脓毒症模型,采用逆转录聚合酶链反应(RT-PCR)和免疫组织化学方法研究ICAM-1和E-选择素的表达;用Hoechest染色评价肺VEC凋亡;用电子显微镜观察肺VEC。结果:脓毒症组大鼠肺ICAM-1 mRNA和ICAM-1蛋白的表达与对照组比较明显增加(P<0.01),脓毒症组ICAM-1 mRNA和ICAM-1蛋白的表达6小时增高,24小时达到高峰;E-选择素表达6小时达高峰,以后逐渐下降,24小时后降至对照组相同水平。脓毒症组肺VEC随着制模时间的延长,坏死和凋亡显著增加(P<0.01),电子显微镜观察也得到证实。结论:脓毒症大鼠肺ICAM-1和E-选择素的表达明显增加,可能导致肺VEC的坏死和凋亡以及急性肺损伤(ALI)和急性呼吸窘迫综合征(ARDS)的发生。
Objective：To observe the changes and significance of pulmonary vascular endothelial cells （VEC）, ICAM-1 and E-selectin in sepsis rats. Methods：60 SD rats were randomly divided into 2 groups：the control group and the sepsis group. The sepsis model was prepared by injection of lipopolysaccharide（4 mg/kg）. The expression of ICAM-1 and E-selectin in pulmonary VEC of rats was detected by reverse transeription-polymerase chain reaction （RT-PCR） and immunohistochemical method. The VEC apoptosis in lung was analyzed with Hoechest-33258 staining. The uhramicrostructure of pulmonary VEC was observed under electron microscope. Results：Compared with the control group, the expression of ICAM- 1 was significantly increased in the sepsis group （ P 〈 0.01 ）, the expression of ICAM- 1 was increased gradually and achieved the peak value at 24 h. The expression of E-selectin was achieved the peak value at 6 h and decreased gradually during 24 h. The apoptosis and necrosis of pulmonary VEC was increased gradually and achieved the peak value at 24 h （P 〈 0.01）. Conclusion：The expression of ICAM-I and E-selectin in sepsis rats is significantly increased, probably leading to both necrosis and apoptosis of pulmonary VEC, resulting in the occurrence of acute lung injury （ALI） and acute respiratory distress syndrome （ARDS）.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2009年第11期1029-1032,共4页
Chinese Journal of Immunology
基金
广东省科技计划资助项目(2005B33001018)
