摘要
目的观察白花蛇舌草诱导HSP70表达对H22肝癌细胞移植瘤小鼠瘤组织细胞凋亡的影响。方法以经过白花蛇舌草和党参处理后的小鼠活体腹水H22肝癌细胞免疫小鼠,用HSP70单抗封闭后分为白花蛇舌草未封闭组与封闭组、党参未封闭组与封闭组以及空白对照组,共5组,每组10只,然后再以培养的H22肝癌细胞攻击小鼠形成移植瘤;采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法原位检测移植瘤小鼠瘤组织细胞凋亡的情况。结果白花蛇舌草组移植瘤小鼠瘤组织细胞核中可见较多棕褐色阳性颗粒,与党参组相比,细胞凋亡数及染色强度明显增多、增强;白花蛇舌草及党参组细胞凋亡的情况与空白对照组比较,差异均具有显著性,尤以白花蛇舌草组更为明显。尽管白花蛇舌草和党参未封闭组与封闭组组间细胞凋亡的差异并无显著性,但白花蛇舌草未封闭组较封闭组具有凋亡数增多和染色强度增强的趋势。结论白花蛇舌草可能通过诱导H22肝癌细胞移植瘤小鼠瘤组织HSP70表达,在一定程度上促进肝癌细胞凋亡而达到抗肿瘤的作用。
Objective To observe the effect of heat shock protein 70 （HSP70） expression induced by Herba Hedyotis Diffusae （HHD） on the apoptosis of H22 hepatoma cells. Methlds Mice were immunized with H22 hepatoma cells from in - vivo ascites of the mice pretreated by HHD and Radix Codonopsis （RC）. Mice were divided into five groups after being blocked with HSP70 monoclonal antibody： non -HHD blocking group, HHD blocking group, non -RC blocking group, RC blocking group and the blank control group, 10 mice in each groups. Then the mice were attacked with cultured H22 hepatoma cells to induce transplanted tumor. The apoptosis in the transplanted tumor was detected by a terminal deoxynucleotidyl transferase- mediated dUTP nick end labeling（TUNEL） method. Results Large amount of brown particles can be observed in the cell nucleus of transplanted tumor mice from HHD groups. Compared with RC groups, number of apoptotic cells and the staining intensity were increased in HHD groups. The apoptosis in HHD groups and RC groups differed from that in the blank control group, and was obvious in HHD groups. The apoptosis exerted a increasing tend in non- HHD blocking group, but the difference was insignificant between non- HHD blocking group and HHD blocking group, and between non - RC blocking group and RC blocking group. Conclusion HHD can promote the apoptosis of H22 hepatoma ceils in transplated tumor through inducing the expression of HSP70 to some extent, and the related mechanism of inducing apoptosis needs further research.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第6期536-539,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省千百十工程优秀人才基金(Q02026)
关键词
白花蛇舌草
HSP70
肝癌
细胞凋亡
Herba Hedyotis Diffusae
Heat shock protein 70
Hepatoma
Apoptosis
