摘要
目的：探讨Ｋ－ｒａｓ，ｐ５３基因表达与人胰腺癌临床生物学行为之间的关系。方法：采用免疫组织化学与斑点杂交方法，检测４８例人胰腺癌组织Ｋ－ｒａｓ，ｐ５３基因表达。结果：胰腺癌组织Ｐ２１ｒａｓ蛋白表达较正常胰腺组织增强（Ｐ＜０．０５），Ⅰ～Ⅱ期较Ⅲ～Ⅳ期表达增强（Ｐ＜０．０５），组织学分级Ｇ１，Ｇ２级较Ｇ３级表达增强（Ｐ＜０．０１），Ｐ２１ｒａｓ蛋白表达阳性患者生存期较表达阴性者延长（Ｐ＜０．０１）；胰腺癌组织Ｐ２１ｒａｓ蛋白与Ｋ－ｒａｓｍＲＮＡ表达呈正相关（ｒ＝０．８９，Ｐ＜０．０１）；胰腺癌组织Ｐ５３蛋白表达较正常胰腺及慢性胰腺炎组织增强（Ｐ＜０．０５），Ⅲ～Ⅳ期胰腺癌较Ⅰ～Ⅱ期Ｐ５３蛋白表达增强（Ｐ＜０．０５），随肿瘤分化程度降低Ｐ５３蛋白表达而增强（Ｐ＜０．０１），Ｐ５３蛋白与ｐ５３ｍＲＮＡ表达呈负相关（ｒ＝－０．４４，Ｐ＜０．０１）。结论：Ｐ２１ｒａｓ与Ｐ５３蛋白表达对估价胰腺癌患者的预后具重要价值；
Objective: To explore the relationship between the expression of the K ras and p53 and the clinicobiological behavior of human pancreatic adnocarcinoma. Methods: The products of the genes of K ras and p53 in 48 human pancreatic adenocarcinomas were detected by using immunohistochemistry and dot blot. Results: It was showed that the expressions of the p21 ras and p53 proteins in pancreatic adenocarcinoma were higher than those in normal pancreatic tissues (P<0.05). The patients with stained positive p21 ras protein had a longer survival than that with stained negative (P<0 01). The patients with stained positive p53 protein had a shorter survival than that with stained negative (P<0 01). The expression of p21 ras protein was correlated with that of K ras mRNA (r=0 89,P<0 01), and the expression of p53 protein had negative correlation with that of p53 mRNA (r=-0.44,P<0 01). Conclusion: It is suggested that the expressions of the proteins of p21 ras and p53 were worthwhile in evaluating the prognosis of the patients with pancreatic adenocarcinoma; the overexpression of K ras mRNA was one of the mechanisms by which the expression of the p21 ras protein was higher in the tissues of pancreatic adenocarcinoma.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1998年第5期470-472,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
全军医药卫生科研基金
