摘要
目的动态研究肾综合征出血热(HFRS)患者不同临床时期T细胞亚群的变化规律,为临床判断病情、评估预后提供免疫学依据。方法在HFRS患者的疾病进展期和恢复期分别应用荧光标记单克隆抗体对患者外周血淋巴细胞进行标记,用流式细胞仪检测T细胞亚群。结果在疾病进展期,CD3+T细胞、CD4+T细胞、CD4/CD8比值均低于恢复期和正常对照组,CD8+T细胞高于恢复期和正常对照组;在疾病进展期,CD4-CD28和CD8-CD28细胞也低于恢复期和正常对照组。结论HFRS患者在疾病进展期存在T细胞亚群功能紊乱和比例失调,在恢复期得以恢复;动态监测T细胞亚群有助于了解机体细胞免疫情况,可以为判断病情、评估预后提供免疫学依据。
Objective Purpose By dynamicly studying the change regularity of T lymphocyte subsets in diferent clinical period of HFRS patients, it is to provide immunological basis to clinical disease state judgement and to prognisis evaluation and estimation. Methods In disease progress period and recover period of HFRS patients, it is to label peripheral blood lymph cells of the patients seporately by employment of fluorescent labels on mono-clone antibody, and to inspect T lymphocyte subsets by utilization of flow cytometry meter. Results In disease progress period, the CD3^＋ T-cells, CD4^＋ T-cells, and CD4/CD8 specific values are all lower than that of the recover period group, and the normal comparison group as well, the CD8^＋ T-cell is higher than that of the recover period and normal comparison groups;in disease progress period,the CD4-CD28 and CD8 - CD28 cells are lower than that of the recover and normal comparison groups either. Conclusion For HFRS patients, the T lymphocyte subsets exist functional disorder and ratio imbalance in the progress period of disease, which have recovered during recover period; the dynamic inspection to T lymphocyte subsets is helpful to the understanding of human organism cell immunity information, which can be providing immunological basis for judging disease state and prognisis evaluation and estimation.
出处
《中国实验诊断学》
北大核心
2009年第11期1587-1588,共2页
Chinese Journal of Laboratory Diagnosis
关键词
肾综合征出血热
T淋巴细胞亚群
免疫应答
Hemorrhagic fever with renal syndrome
T lymphocyte subsets
immune response
