摘要
目的探讨内毒素脑损伤大鼠模型的顶叶皮质、海马组织、脑脊液(CSF)和血浆中脑红蛋白(neu-roglobin,Ngb)的表达变化及其意义。方法随机选用SD大鼠140只,分为1个对照组(n=20)和6个内毒素干预组(n=20)。内毒素干预组向大鼠脑池内注射内毒素0.1mg/kg体重,对照组注入等容量生理盐水。于注射内毒素后3、6、12、24、48及72h收集血浆、CSF,并处死大鼠留取顶叶皮质和海马组织。应用酶联免疫吸附法、免疫印迹法和免疫组织化学法检测Ngb的表达变化;应用干燥法检测鼠脑含水量。结果注射内毒素后,鼠脑含水量明显高于对照组(P<0.01),48h达峰值。酶联免疫吸附法测量顶叶皮质、海马组织、CSF和血浆中Ngb含量,显著高于对照组(P<0.01),48h达峰值。免疫印迹法和免疫组织化学法的结果和上述结果一致。结论在内毒素所致脑损伤中Ngb表达上调且与内毒素的注入时间相关,Ngb表达上调是机体内源性神经保护机制之一。
[ Objective ] To study the changes of neuroglobin （Ngb） in rats during the process of brain edema induced by endotoxin. [Methods] 140 aduh SD rats were randomly divided into one control group （n =20） and six operation groups （n =20）. In the operation groups, 0.1 mg endotoxin was intraventricularly injected in every one kilogram weight of 120 rats, while the same volume of normal saline was injected in 20 rats as control. The expression of Ngb in the plasma, the CSF, the hippocampus and the parietal cortex over different periods were detected by enzyme-linked immunpsorbert assay, Western blotting and immunohistoehemieal method. The brain water content was measured by dry method. [Results] After endotoxin injection, the brain water levels were significantly higher （P 〈0.01）, and peaked at 48 h. The expression of Ngb was significantly higher than those of control group （P 〈0.01）, and peaked at 48 h. [ Conclusions ] The expression of Ngb was overexpressed during the process of brain edema induced by endotoxin in the rats, and changed in a time-dependent manner. It was one of the endogenous nerve protective mechanisms.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第22期3368-3373,共6页
China Journal of Modern Medicine
基金
国家自然科学基金(No:30270437)
教育部春晖计划(No:2003-26)
