摘要
目的观察早期应用前列腺素E_1(PGE_1)对家兔单侧后肢电烧伤后局部股静脉血中的红细胞变形性、血小板聚集率和血管内皮素-1以及损伤肌肉组织中的ATP含量变化的影响。方法采用家兔单侧后肢电烧伤模型,分别用激光衍射法、放射兔疫法和高压液相色谱等方法对实验指标进行测定。结果肢体电烧伤后,局部深静脉血中的红细胞变形性(RCD)、血小板聚集率(PAR)和血管内皮素-1(ET-1)的含量均有明显改变。早期应用PGE_1可显著改善电烧伤组织深静脉血中的RCD,降低PAR以及ET-1的含量,并提高损伤肌肉组织中的ATP含量,其与未治疗组及对侧健肢相比均有非常显著差别。结论肢体高压电烧伤后,局部血液中有明显的血液流变学变化,早期应用PGE_1可减轻电烧伤组织中血液的超凝血状态,预防大血管继发血栓形成,保护间生态组织。
Objective The study was designed to investigate the changes in some rheological pa- rameters and ET-1 in venous blood following electrical burns. Methods One of the hind limbs of rab- bits was subjected to 3000V,2.5A charge of alternative electric current for 3 seconds. Rheological pa- rameters such as red cell deformability(RED) and platelet aggregation rate (PAR), as well as endothe- lin-1 (ET-1) in venous blood drained from electrical burn wounds and contents of ATP in damaged mus- cle were measured at time intervals of 2h,24h-,48h and 72h postburn. Results The changes in the pa- rameters mentioned above were significant following electrical injury. The administration of PGEI showed an advantageous effect in prevention of thrombosis and muscle 'progressive necrosis' through improvement in RCD, reduction of PAR and the content of ET-1 in local circulation. Conclusion RCD, PAR and the content of ET-1 in local circulation changed significantly following electric injury and may play an important role in the process of 'progressive necrosis'. The potential use of PGE1 in the treat- ment of electrical injury is proposed.
