摘要
目的：为囊虫的诊断提供简便有效的方法。方法：猪囊尾蚴ｃＤＮＡ表达文库中筛选出的β－半乳糖苷糖－猪囊虫ｃＤＮＡ编码的融合蛋白为抗原，进行简化的Ｗｅｓｔｅｒｎｂｌｏｔ分析。结果：四个克隆的融合蛋白（ｃＣ１、ｃＣ２、ｃＰ１和ｃＨ１）联合使用，检测１２４例人囊虫病血清中的抗囊尾蚴ＩｇＧ抗体，阳性率达９３．７％，三个克隆的融合蛋白（ｃＣ１、ｃＣ２、和ｃＰ１）联合使用，检测３８例猪囊虫病血清中的相应抗体，阳性率达１００％，均高于各克隆融合蛋白单独使用的阳性率，且与其他寄生虫病血清无交叉反应。结论：以融合蛋白为抗原所进行的Ｗｅｓｔｅｒｎｂｌｏｔ分析具有高度灵敏性和特异性，且简便易操作。
AIM:To provide simple and useful method for the immunodiagosis of cysticercosis.METHODS:Fusion protein of β galactosidase Cysticercus cellulosae cDNA was analysed with simplified Western blot.RESULTS:The positive rate was 93.5% when detecting 124 cases of human cysticercosis sera with four clones (cC1,cC2,cP1 and cH1) and 100% when detecting 38 cases of porcine cysticercosis sera with three clones (cC1,cC2 and cP1),being higher than those using monoclone fusion protein alone.Moreover, the fusion proteins did not react with other parastitosis sera.CONCLUSION:Simplified Western blot analysis using recombinant fusion protein as antigen was highly sensitive and specific and easy to be operated.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1998年第5期360-363,共4页
Chinese Journal of Parasitology and Parasitic Diseases
