摘要
目的研究ＭＲＬ／ｌｐｒＴｈｙ１．１小鼠中Ｔ细胞的异常、异常Ｔ细胞的起源及其与淋巴腺病理的关系。方法取出生后不同时期ＭＲＬ／ｌｐｒＴｈｙ１．１小鼠及正常Ｃ５７ＢＬ／６小鼠的造血淋巴组织，制备单细胞悬液并用流式细胞技术检测其细胞表面标记，然后以ｔ检验进行统计学处理。结果发现ＭＲＬ／ｌｐｒＴｈｙ１．１小鼠中存在着一个异常的、与淋巴腺病理密切相关的Ｔ细胞亚群，其表型为Ｔｈｙ１．１－ＴＣＲαβ＋Ｌｙ５＋，而且这一细胞亚群随着年龄增长而迅速增加，至１９周龄，其可以占淋巴结细胞总数的８０％。同时，淋巴结及脾脏中传统Ｔ细胞（Ｔｈｙ１．１＋ＴＣＲαβ＋Ｌｙ５－）随着Ｔｈｙ１．１－ＴＣＲαβ＋Ｌｙ５＋细胞的增加而逐渐减少。结果还表明，这一细胞亚群既不在胸腺也不在骨髓中分化成熟，但这一细胞亚群起源于某些异常骨髓造血细胞。结论ＭＲＬ／ｌｐｒＴｈｙ１．１小鼠中存在着一个起源于某些异常骨髓造血细胞的表型为Ｔｈｙ１．
Objective To investigate T cells' abnormality,the source of the abnormal T cells and the relationship between abnormal T cells and lymphadenopathy in MRL/lpr Thy1.1 mice. Methods The lymphohemopoietic cells were obtained from various week old MRL/lpr Thy1.1 mice or normal mice.Then the surface markers of these cells were examined with FCM.Student t test was used to assess the statistical significance. Results In the experiments,an abnormal novel T lymphocyte subpopulation in MRL/lpr Thy1.1 mice was identified .The pattern of the subpopulation was of Thy1.1 -TCRαβ +Ly5 +.This subpopulation was associated with murine lymphadenopathy and increased sharply with ageing.The percentage of it in lymph node was about 80% in 19 week old mice.In contrast,the conventional T lymphocytes(Thy1.1 +TCRαβ +Ly5 - T cells) in the lymph node and spleen decreased sharply while the abnormal subpopulation increased.By means of bone marrow transplantation,we found that this novel T cell subset was derived from some abnormal hemopoietic cells,although its differentiation sites remained obscure. Conclusions There exists Thy1.1 -TCRαβ +Ly5 + T lymphocyte subpopulation in MRL/lpr Thy1.1 mice.This novel T cell subset has been derived from some abnormal hemopoietic cells.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1998年第6期464-468,共5页
Chinese Journal of Microbiology and Immunology
关键词
自身免疫疾病
T淋巴细胞亚群
骨髓移植
Autoimmune diseases \ \ T lymphocyte subpopulation\ \ Bone marrow transplantation\ \ Lymphadenopathy
