摘要
【目的】筛选新疆小芦苇青贮中优良的乳酸杆菌。【方法】研究将从自制的新疆小芦苇青贮中分离出乳酸杆菌进行鉴定,对鉴定出的菌株进行产酸试验,确定新疆小芦苇青贮中的优势菌株。【结果】试验共分离得到了11株乳酸杆菌,根据《伯杰氏细菌鉴定手册》(第八版)中的种属表述结果,经鉴定2株(Hz2-1和Hz2-2)为戊糖乳杆菌(L.pentosus),2株(Hz3-1和Hz3-2)为植物乳杆菌(L.plantarum),2株(Zar2-8和Zar2-9)为牛粪乳杆菌(L.dung),2株(Zar8-3和Zar8-4)为短乳杆菌(L.brevis),3株(Gsr1-1、Hz1-1和Zar9-4)为德氏乳杆菌保加利亚亚种(L.delbrueckiisubsp.bulgaricus)。【结论】从不同菌株发酵产酸试验的结果来看,发现Hz2-1,Hz3-1具有较强的产酸能力,pH最低可达3.50,是新疆小芦苇青贮发酵过程中的主要产酸菌株。
【Objective】Xinjiang small reed distributed widely and which is good forage resources of ruminants.【Method】This study will isolate and identify Lactobacillus from the self-regulating Xinjiang small reed silage.The trains were acidogenic tests that identified the advantages trains of Xinjiang small reed silage.11 strains of Lactobacillus were isolated from the self-regulating reed silage of different origin.According to the criteria and description of Bergey＇s manual of systematic bacteriology（The eighth edition）,which include two strains Lactobacillus pentosus（Hz2-1,Hz2-2）,two strains Lactobacillus plantarum（Hz3-1,Hz3-2）,two strains lactobacillus dung（Zar2-8,Zar2-9）,two strains Lactobacillus brevis（Zar8-3,Zar8-4）,three strains Lactobacillus delbrueckii subsp.bulgaricus（Gsr11,Hz1-1,Zar9-4） were identified.Based on a strain for the fermentation acid test,it was found that a strong acid production capacity were observed from HZ2-1,HZ3-1,the pH up the minimum could reach 3.50,which was the main strains from fermentation process of Xinjiang small reed.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2009年第6期1327-1331,共5页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区重大专项项目"奶牛产业技术开发与集成示范"(200731134)
国家科技支撑计划项目"西北农区生态型奶业生产技术和奶牛性控技术研究与开发"(2006BAD04A11-06)
农业部奶产业技术体系项目
关键词
新疆小芦苇
青贮
乳酸杆菌
分离鉴定
Xinjiang small reed
silage
Lactobacillus
isolation and identification
