摘要
目的观察丁酸梭菌对实验性结肠炎小鼠肠黏膜趋化因子RANTES和单核细胞趋化蛋白(MCP)-1表达的影响,并探讨其可能的作用机制。方法采用2.5%的葡聚糖硫酸钠(DSS)建立小鼠结肠炎模型。55只BALB/c小鼠随机分为健康对照组,阴性对照(生理盐水)组,阳性对照(美沙拉嗪)组,模型对照组,丁酸梭菌108CFU/mL组。用疾病活动指数(DAI)评分和病理组织学积分检测干预组的疗效;免疫组化法检测结肠组织中趋化因子RANTES和MCP-1蛋白的表达;反转录-聚合酶链反应(RT-PCR)法检测结肠组织中RAN-TES、MCP-1基因的表达。结果丁酸梭菌可改善小鼠一般情况,降低DAI积分,与模型对照组、阴性对照组相比,RANTES和MCP-1蛋白、基因的表达量均减少(P<0.05);其效果与美沙拉嗪相当。结论丁酸梭菌对急性期实验性结肠炎小鼠有治疗作用,其部分机制可能与通过减少结肠黏膜趋化因子RANTES和MCP-1的表达有关。
Objective To investigate the effect of Clostridium butyricum on expression of chemokines RAN- TES and MCP-1 in murine intestinal mucosa with ulcerative colitis and its potential mechanism. Methods Dextran sulphate sodium （DSS） was used to induce BALB/c mice UC model. Fifty five mice were randomly divided into five groups as follows： normal control group, negative control group （NS） , positive control group （mesalazine）, model control group, Clostridium butyricum 108 CFU/ml group. Disease activity index （DAD , histological injury score were used to assess the efficacy of intervention groups; ehemokines RANTES and MCP-1 expression in co- lonic mucosa were measured by immunohistochemistry （IHC）; RANTES and MCP-1 mRNA expression were measured by reverse transcription polymerase chain reaction （RT-PCR）. Results Clostridium butyricum could improve the general situation in mice and lower DAI scores; The expression of RANTES and MCP-1 （the level of protein and mRNA） were both decreased （P^0.05） in Clostridium butyricum group, compared with model con- trol and negative control groups; however, its effect is similar to mesalazine. Conclusion Clostridium butyricum had a therapeutic effect on the acute UC mice effectively;Its mechanism was possibly related to lower the expres- sion of chemokines RANTES and MCP--1 in colonic mucosa.
出处
《山西医药杂志（上半月）》
CAS
2009年第12期1075-1078,共4页
Shanxi Medical Journal
基金
山西省回国留学人员科研资助项目
关键词
结肠炎
溃疡性
丁酸梭菌
趋化因子类
Clostridium butyricum
Ulcerative colitis
Chemotactic factors
