摘要
目的采用HPLC法测定四季感冒片中连翘苷的含量。方法采用外标一点法,Diamonsil ODS1 C18色谱柱,乙腈-水(25∶75)为流动相,流速为1.0 mL/min,检测波长为277 nm。结果连翘苷在0.112 8～0.902 4μg范围内呈良好的线性关系,回归方程为Y=5.795 68X+1.843 19,r=0.999 3,平均加样回收率为98.6%,RSD=1.82%(n=6)。结论本法简便、准确,专属性强,测定结果重复性好,为四季感冒片中连翘苷定量分析提供了有效的方法。
Objective To establish the method for detemining the content of Phillyin in Siji Ganmao Pian by HPLC.Methods Diamonsil ODS1 C18 column was used with acetonitrile-water（25∶75） as the mobile phase,detection wavelength as 277 nm and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.1128～0.9024 μg for Phillyin and linear equation was Y =5.795 68X ＋1.843 19,r =0.999 3.The average recovery was 98.6% and RSD was 1.82%（n =5）.Conclusion This method was simple,accurate and proper, the reduplication of the result was good. The method can be provide for quantitative analysis of Phillyin in Siji Ganmao Pian.
出处
《中国中医药信息杂志》
CAS
CSCD
2009年第10期51-52,共2页
Chinese Journal of Information on Traditional Chinese Medicine
