摘要
【目的】研究紫茎泽兰F3′H在次生代谢途径中的功能。【方法】构建了F3′H过表达载体,并将其转入烟草体内,进一步通过半定量RT-PCR和Real-timePCR检测F3′H的表达情况。【结果】F3′H在烟草体内能正常表达,但在开花后则受到抑制,表现为转F3′H烟草花色较野生型变淡,F3′H表达量低于野生型表达量。【结论】F3′H与次生代谢途径中花色素的形成密切相关,且转入烟草体内时,其表达与烟草内源基因表达相互影响,导致烟草的花色变淡。
【Objective】 The objective of this study is to identify the function of F3′H gene from Eupatorium adenophorum in the secondary metabolism pathway.【Method】 The over-expression vector of F3′H gene was constructed,and transformed into tobacco,furthermore,semi-quantitative PCR and real-time PCR were used in the study to detect the status of F3′H gene.【Result】 Although F3′H gene could be expressed in the transgenic tobacco normally,it was suppressed after flowering.The color of flowers became less bright in transgenic tobaccos than in wild-type tobaccos,at the same time,the level of F3′H gene was lower in transgenic tobacco than in wild-type tobaccos.【Conclusion】 The result suggested that F3′H gene has close relation with the formation of anthocyanins in the secondary metabolism pathway.Expression of F3′H gene affected with endogenous F3′H gene each other after it was transferred into tobaccos,which lead to slight loss of color in flowers.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第12期4182-4186,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金(30771422)
国家重点基础研究发展计划("973")项目(2009CB119201)
关键词
类黄酮3′-羟化酶
过表达
共抑制
flavonoid 3′-hydrolyase
over-expression
co-suppression
