摘要
采用室内离体接种和室外孢子喷雾接种方法，对3株梨黑斑病病原菌的生物学特性和致病性进行了观察。结果表明：供试各菌株在PSA培养基上培养，平均日生长速率、产孢量、菌落颜色及菌落厚度差异显著；各病原菌菌株在光照、黑暗和12h光暗交替培养时，日生长速率和产孢量有明显差异；黑斑病病原菌最适生长和产孢培养基为PSA；孢子萌发最适pH值8～10；菌株A和菌株H的致病力显著强于菌株C，但这2个菌株间的致病性无显著差异；A、C、H3种菌株混合接种时，可使梨叶片表现自然发病时的典型病斑。
Biological characteristics and pathogenicity of three strains of Alternaria alternate （Fr.） Keissl which caused pear black spot disease were studied. The results indicated that the daily growth rate, the quantity of produced spore, the color and the thickness of colony of the three strains were significantly different in PSA medium. The daily growth rate and the quantity of produced spores of the three strains were also significantly different. The optimum growth and producing spores media was PSA media. The optimum pH value of spores germination was 8- 10. Their pathogenicity was tested on in vitro pear leaves and field plants. The results showed that the pathogenicity of A strain and H strain were significantly stronger than that of C strain, but there was no significant difference between A and H. The representative disease spot which occurred in natural condition exhibited when the pear leaves were inoculated with the mixture of the three strains.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2009年第6期680-684,共5页
Journal of Huazhong Agricultural University
基金
国家科技基础条件平台项目(2006DKA21002-24)
现代农业产业技术体系建设项目(nycytx-29-23)
湖北农业科技创新专项资金(2007-620-005-03-03)资助
关键词
梨黑斑病
生物学特性
致病性
病情指数
pear black spot disease
biological characteristics
pathogenicity
exponent of disease
