摘要
目的:观察槐角总黄酮对高脂血症大鼠的降血脂及抗脂质过氧化作用.方法:用高脂饲料喂养大鼠4wk,建立实验性高脂血症大鼠模型,给予槐角总黄酮40,80,160mg/kg体质量,连续6wk,以绞股蓝总皂苷为阳性对照,检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量;测定血液黏度、肝脏丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力及总抗氧化能力(T-AOC)的变化;逆转录-多聚酶链反应(RT-PCR)检测肝低密度脂蛋白受体(LDL-R)基因mRNA的表达.结果:槐角总黄酮能降低高脂血症大鼠血清TC,TG,LDL-C水平(P<0.05或P<0.01),升高HDL-C水平(P<0.05);降低全血黏度,使肝组织MDA水平降低,SOD及T-AOC活力升高,促进LDL-R基因mRNA的表达.结论:槐角总黄酮对高脂血症模型大鼠脂代谢紊乱具有较好的调节作用,可能是通过降低血液黏度、抗脂质过氧化,对肝LDL-R基因mRNA表达的调控来实现其降脂作用.
AIM：To explore the antioxidation and the serum lipid lowing effects of total flavonoid from fructus sophorae（TFFS）on experimental hyperlipidemic rat.METHODS：To establish hyperlipoidemic rats model with high lipid diet for 4 weeks and then give experimental treatment with TFFS with dose of 40,80,120 mg/kg of body weight for 6 weeks.Gypenosides was also used as a positive control,the serum were collected to measure the total cholesterol（TC）,triglyceride（TG）,high density lipoprotein cholesterol（HDL-C）,low density lipoprotein cholesterol（LDL-C）,whole blood viscosity was measured,and the liver tissue was also collected for determining malonaldehyde（MDA）content and the activities of superoxide dismutase（SOD）and total antioxidation capacity（T-AOC）.The expression level of low density lipoprotein receptor（LDL-R）gene mRNA in liver were determined by reversed transcription polymerase chain reaction（RT-PCR）method.RESULTS：Compared with model group,TFFS could decrease TC,TG and LDL-C level（P〈0.05 or P〈0.01）,increase HDL-C level（P〈0.05）in blood,They had the effects of decreasing the whole blood viscosity and the secretion of MDA,increase the activities of SOD and T-AOC,and up-regulation of the expression level of LDL-R gene.CONCLUSION：TFFS has the activity of modulating lipid metabolite of high-lipid rat.Its mechanism may be related with recede blood viscosity,anti-lipoperoxidation and up-regulationof the expression of LDL-R gene in mRNA level.
出处
《第四军医大学学报》
北大核心
2009年第22期2677-2681,共5页
Journal of the Fourth Military Medical University
关键词
槐角
黄酮
脂代谢
高脂血症模型
肝低密度脂蛋白受体
fructus sophorae flavonoid lipometabolism hyperlipisdemia model lipoprotein liver low density lipoprotein receptor
