摘要
目的探讨糖耐量减低(IGT)人群血小板活化状态及影响因素。方法选择IGT组33例、糖尿病组和正常对照组各30例为研究对象。采用流式细胞术(FCM)检测血小板膜蛋白CD62P、血小板激活复合物(PAC-1)阳性率和平均荧光强度(MFI);电阻抗法测定血小板数目(count)、血小板平均体积(MPV)、血小板分布宽度(PDW)及大血小板比率(P-LCR)等参数及总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、极低密度脂蛋白胆固醇(VLDL-C)、空腹血糖(FPG)和空腹胰岛素(FIns)。结果IGT组、糖尿病组CD62P%(P<0.01)、CD62PMFI(P<0.05)、PAC-1%(P<0.01)、MPV(P<0.05)、PDW(P<0.05)及P-LCR%(P<0.05)均较正常对照组升高;IGT组与糖尿病组之间差异无统计学意义(P>0.05)。多元回归分析显示,IGT组与CD62P密切相关的因素依次为P-LCR、LDL-C、TC和体质量指数(BMI);与PAC-1相关的因素依次为P-LCR、MPV及DBP。结论IGT人群存在与糖尿病同等程度的血小板活化;LDL-C、TC、DBP及BMI是影响血小板活化的因素。
Objective To investigate the state of platelet activation and its influencing factors in subjects with impaired glucose tolerance（IGT）.Methods 33 patients with IGT were enrolled in this study.30 DM and healthy persons served as the controls.Percentage and mean fluorescence intensity of CD62P,PAC-1 as indexes of platelet activation were measured using whole blood flow cytometry（FCM）.Changes of count of blood platelet,mean platelet volume（MPV）,platelet distribution width（PDW） and platelet-large cell ratio（P-LCR）were analyzed with a blood cell counter.TC,TG,HDL-C,LDL-C,VLDL-C,FPG and FIns were simultaneously measured.Results IGT and subjects with diabetes had higher levels of CD62P%（P〈0.01） and CD62MFI（P〈0.05） and PAC-1%（P〈0.01） compared with the NC group.CD62P%,CD62MFI and PAC-1% had no statistical differences between the IGT and DM groups（P〉0.05）.By stepwise regression analysis,CD62P in the IGT group had relation to P-LCR,LDL-C,TC and body mass index（BMI） independently.P-LCR,MPV and DBP were contri-butors to PAC-1.Conclusion There is the same degree of platelet activation in subjects with IGT,and DM.LDL-C,TC,DBP and BMI are contributors to CD62P and PAC-1 expressions on platelet in IGT.
出处
《山东大学学报（医学版）》
CAS
北大核心
2009年第11期9-13,共5页
Journal of Shandong University:Health Sciences
基金
泰安市科技发展计划项目(200083016)
