摘要
目的:探讨红霉素对哮喘大鼠磷酸化P38及Th2类细胞因子的影响。方法:分离培养正常对照组、哮喘组、红霉素处理组大鼠的外周血单核细胞(PBMCs)。分别采用ELISA方法检测细胞上清液中IL-4、IL-5、IL-13浓度。逆转录聚合酶链反应(RT-PCR)、Western blotting技术检测PBMCs P38mRNA、P38磷酸化蛋白的表达。结果:哮喘组PB-MCs P38mRNA、P38磷酸化蛋白表达水平及细胞上清液中IL-4、IL-5、IL-13的浓度较正常对照组显著升高(P<0 .05) ,红霉素处理组P38 mRNA、磷酸化蛋白表达水平及细胞上清液中IL-4、IL-5、IL-13的浓度较哮喘组显著降低(P<0 .05)。通过直线相关分析发现,PBMCs P38磷酸化蛋白与细胞上清液中IL-4、IL-5、IL-13的表达水平之间均呈显著正相关(r分别=0 .75、0 .82、0 .70 ,P均<0 .05)。结论:P38可能参与支气管哮喘的病理生理过程。红霉素可能通过作用于P38这一靶点发挥其免疫调节作用。
AIM：The purpose of this study is to explore the effects of erythromycin on the expressions of phosphorylation P38 and Th2 cytokines in asthmatic rats.METHODS：Thirty male Sprague-Dawley adult rats were randomly divided into normal,asthma and erythromycin-treated group （n=10 for each group）.Peripheral blood monouclear cells（PBMCs）of rats were collected and cultured in each group.The concentrations of IL-4,IL-5 and IL-13 in cell supernatant were detected by ELISA.The mRNA and phosphor protein of P38 in PBMCs were detected by reverse transcription polymerase chain reaction （RT-PCR） and Western blotting （WB）,respectively.RESULTS：The expression of mRNA and phosphor protein of P38 in PBMCs and the concentrations of IL-4,IL-5 and IL-13 in cell supernatant were higher in asthmatic group than those in normal group （P〈0.05）.Compared with the asthmatic group,the above indexes were significantly decreased in erythromycin-treated group （P〈0.05）.There were positive correlations between the expression of phosphor-P38 in PBMCs and the concentrations of IL-4,IL-5 and IL-13 in cell supernatant （r=0.75,0.82 and 0.70,all P〈0.05）.CONCLUSION：The P38 may be involved in the pathogenesis of bronchial asthma.Erythromycin playing its role in the treatment of asthma may be achieved by acting on the P38.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2009年第10期1151-1155,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
温州市科学技术局资助项目(Y2006A036)
关键词
哮喘
外周血单个核细胞
P38
红霉素
asthma
peripheral blood mononuclear cell
P38
erythromycin
