摘要
目的研究皮下注射毒扁豆碱(PHY)引起低温反应与内源性精氨酸加压素(AVP)的关系。方法用无线遥测技术连续记录25℃环境温度中雌性大鼠的体核温度和活动,用数字体温计测量尾部皮肤温度,每次间隔30min。皮下注射 PHY(0.25mg/kg)引起大鼠低温期,分别给动物注射中枢胆碱能阻断剂东莨菪碱(1mg/kg,sc)和 AVP V_1受体阻断剂(30μg/kg,ip)。注射 PHY 50min 后,测量血浆中 AVP 的含量。结果皮下注射 PHY 可导致体温快速降低,同时伴有尾部皮肤散热反应明显增加。在给 PHY 50min 后,血浆中 AVP 的含量明显升高。东莨菪碱和 AVP V_1受体阻断剂可明显阻断 PHY 引起的低温反应。结论内源性退热物质 AVP 参与 PHY 引起的低温反应过程。这可能是易逆性抗胆碱酯酶药(如 PHY)引起低温反应的一个新机制。
Objective To study the subcutaneous injection of physostigmine （PHY） inducing hypothermic response and its relation-ship with endogenous arginine vasopressin （AVP）. Methods Core temperature and motor activity were monitored by telemetry in female rats maintained at an ambient temperature of 25℃. Tail skin temperature was measured at 30 min intervals with digital thermometer. The central cholinergic antagonist, scopolamine（1mg/kg） and AVP V1 receptor antagonist were administered during the period of PHY（0.25mg/kg） induced hypothermia. Plasma AVP concentration was measured at 50 min after administration of PHY. Results Subcuta- neous injection of PHY led to a rapid reduction in core temperature concomitant with a marked elevation in the heat loss from the tail. The hypothermic response of PHY was blocked by scopolamine and AVP V1 antagonist. Plasma AVP concentration increased markedly at 50 min after PHY. Conclusion The results suggest that endogenous AVP could be involved in PHY -induced hypothermic processes. This may be a novel mechanisms of action for a reversible anticholinesterase drug （such as PHY）-induced hypothermia.
出处
《医学研究杂志》
2009年第12期25-28,共4页
Journal of Medical Research
基金
国家自然科学基金资助项目(30870901)
