摘要
观察烟草特异亚硝胺ＮＮＫ在诱发人胎肺组织（ＨＦＬＴ）癌变中的启动作用。结果显示：（１）在０～５００μｇ／ｍｌ范围内，ＨＬＦＴ中８ＯＨｄＧ含量随ＮＮＫ剂量的增加而增高；（２）５００μｇ／ｍｌＮＮＫ处理组ＨＬＦＴＤＮＡ与Ｎｅｕ基因共转染后所得Ｇ４１８抗性细胞可在裸鼠体内形成纤维肉瘤，且细胞形态呈多角形，提示ＮＮＫ所致ＨＬＦＴＤＮＡ损伤使其获得了转化Ｒａｔ１细胞的潜能。说明ＮＮＫ在诱导ＨＬＦＴ癌变中发挥启动作用。
The initiation of carcinogenesis in human fetal lung tissues(HFLT) induced by tobaccospecific nitrosamine,NNK,was in vestigated.We observed that(1) the leve of 8OHdG in HFLT increased with increasing concentrations of NNK in the range of 0～500μg/ml;(2)the NNKtreated HFLT(500μm/ml)and Neu gene have the ability to form fibrosarcoma with epithelial morphology in nude mice,indicating that the cells in HFLT with DNA damage induced by NNK acquired the potential to transform the Rat1 cells.Above results showed NNK acts as an initator in carcinogenesis of HFLT and it might provide a clue to seek the molecular marker in the early stage of human lung carcinoma.
关键词
烟草特异亚硝胺
肺癌启动模型
人胎
肺细胞癌变
Tobaccospecific nitrosamine Initiative model of lung caicinoma Humaan fetal lung tissue 8OHdG Molecular marker
