摘要
以巴戟天的根为原料,经热水浸提、Sevag法除蛋白、乙醇沉淀和DEAE-Sepharose CL-6B离子交换柱层析,得到一种水溶性的巴戟天多糖(MOPI-3).通过UV、IR、NMR、GC-MS、高碘酸氧化、Smith降解和甲基化等物理化学方法对MOPI-3的纯度、理化性质和组成结构进行表征.结果表明,MOPI-3分子量为36061,是一种由阿拉伯糖、半乳糖和葡萄糖组成的杂多糖,以α-1,3-吡喃葡萄糖和α-1,4-吡喃半乳糖为主链,平均每5个葡萄糖连接一个半乳糖,每个重复单元具有一个支链,支链由3个呋喃阿拉伯糖以α-1,3-键型组成,连接在主链葡萄糖的6位碳上,MOPI-3含有乙酰基,连接在主链半乳糖的2位碳原子上.
Morinda officinalis How is a rare Chinese traditional herbal medicine. A water soluble polysaccharide（MOPI-3） was obtained from Morinda officinalis How, by hot water extraction, deproteination with Sevag method, precipitation with ethanol, and fractionation through DEAE-Sepharose CL-6B ion exchange chromatography. Using UV, IR, NMR, GC-MS, periodate oxidation, Smith degradation and methylation reaction, the primary structure of MOPI-3 was identified. The results show that, MOPI-3 is highly purified with average molecular weight of 35309 determined by high performance gel permeation chromatography. And it is a heteropolysaccharide composed of Ara, Gal and Glc. The backbone of MOPI-3 was linked by α-1,3-pyran-glucose and α-1,4-pyran-galactose, and the molar ratio of glucose to galactose was 5：1. There was one side chain composed by three α-1,3-furan-arabinoses linked onto C6 of glucose on backbone in each repeat unit, and the O-acetyl group was linked onto C2 of galactose.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2009年第12期2391-2395,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20436020)
广东省科技攻关项目(批准号:2003C104025)资助
关键词
巴戟天
多糖
分离
结构表征
Morinda officinalis How
Polysaccharide
Isolation
Structure characterization
