摘要
目的:研究定心方对缺血再灌注大鼠心肌MAPK通路的调节作用。方法:将40只雄性Wistar大鼠随机分为假手术组、模型组、定心方高、中、低剂量组,应用结扎(15min)和松开(30min)左冠状动脉前降支的方法制作缺血再灌注模型。应用免疫印迹技术检测MAPK通路中磷酸化ERK1/2、JNK1/2、P38蛋白的表达,并记录再灌注过程中Ⅱ导联心电图。结果:与模型组相比,其余各组的pJNK1/2和pP38表达及心律失常评分显著降低(P<0.05);定心方用药组的pERK1/2蛋白表达显著高于假手术组和模型组(P<0.001),而两组间无显著差异(P>0.05)。结论:定心方可以通过调节MAPK通路的激活,减轻大鼠心肌缺血再灌注损伤。
Objective:To study effect of DingXin Recipe on MAPK pathway of myocardial ischemia reperfusion Injury in Rats. Methods :40 wistar rats were randomly divided to five groups : sham operation group, model group,low dose ,middle dose and high dose DingXin Recipe groups . The model of myocardial ischemia reperfusion in rats were established by ligating the left coronary arteries for 15min and then loosing for 30min. The expression of pERK1/2 , pJNK1/2 , pP38 were detected by western blotting . Ar- rhythmia was scored according to Ⅱ - lead ECG. Results : Compared with model group ,The level of pJNK1/2 and pP38 and arrhythmia score was significantly decreased (P 〈 0.05 ). The level of pERK1/2 was higher in DingXin Recipe groups than other groups (P 〈 0. 001 ), and sham operation group was same with modle group (P 〉 0. 05 ). Conclusion:Myocardial ischemia reperfusion in- jury can be alleviate. The mechanisms may be that MAPK pathway was influenced by DingXin Recipe .
出处
《辽宁中医杂志》
CAS
北大核心
2009年第12期2191-2193,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然基金资助项目(30572435)
关键词
定心方
心肌缺血再灌注
丝裂原活化蛋白激酶
DingXin Recipe
myocardial ischemia reperfusion
mitogen - activated protein kinase