摘要
研究并建立了测定贝类中多氯联苯残留的气相色谱-串联质谱（GC—MS／MS）方法。通过正己烷和丙酮的混合溶液超声提取，硅胶柱净化，旋转蒸发浓缩，采用GC—MS／MS多反应监测（MRM）方式测定，可同时对贝类中的7种指示性多氯联苯进行定性和定量。研究结果表明：7种指示性多氯联苯的检出限（S／N=3）为0．0066～0．0098μg／kg，定量限（S／N=10）为0．02μg／kg；在0．1～50．0ng／mL时峰强度与质量浓度的线性关系良好（r〉0．99）。方法的平均回收率为73％～113％。该法简便快捷，降低了分析成本，在一定程度上实现了持久性污染物的快速检测和确证。
A method for determining residues of seven polychlorinated biphenyls （7 PCBs） in seashell was developed by gas chromatography-tandem mass spectrometry. The sample was extracted with blended solution of hexane and acetone, cleaned-up by column of silica gel, and concentrated with a rotary evaporator. The mass spectrometer was operated using multiple reaction monitoring （MRM）for qualitative and quantitative analysis of 7 PCBs in seashell at the same time. The results showed that the limits of detection for 7 PCBs were 0. 0066- 0. 0098 μg/kg （S/N= 3）, and the limits of quantitation were 0.02 μg/kg （S/N=10）. The method was linear over the range from 0.1 ng/mL to 50 ng/mL with correlation coefficients 〉0. 99, and the average recoveries for 7 PCBs were between 73% and 113%. The method is simple, fast and low cost, which facilitates the fast analysis of the permanent contaminations.
出处
《分析科学学报》
CAS
CSCD
北大核心
2009年第6期647-651,共5页
Journal of Analytical Science
基金
农业部948资助项目(No.2006G56)
国家科技支撑计划项目(No.2007BAD62B02)
国家863计划项目(No.2008AA100805)
关键词
多氯联苯
贝类
气相色谱-串联质谱法
Polychlorinated biphenyls
Seashell
Gas chromatography-tandem mass spectrometry
